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Anti-ITGAL Antibody EP1285Y
Also for ITGAL (NM_002209)
|A synthetic peptide corresponding to residues near the C-terminus of human LFA-1a was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||IHC-Fr: Use at an assay dependent concentration; WB: 1:1000 - 1:10000; IP: 1:100; FC: 1:80 - 1:100; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100 - 1:250; Blocking: Use at an assay dependent concentration
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide) (ITGAL), transcript variant 1|
|CD11A; LFA-1; LFA1A|
|In the immune system, integrins have essential roles in leukocyte trafficking and function. These include immune cell attachment to endothelial and antigen-presenting cells, cytotoxicity, and extravasation into tissues (1). Integrin adhesion receptors transduce signals that control complex cell functions which require the regulation of gene expression, such as proliferation, differentiation and survival. Their intracellular domain has no catalytic function, indicating that interaction with other transducing molecules is crucial for integrin-mediated signaling. JAB1 (Jun activation domain-binding protein 1), a coactivator of the c-Jun transcription factor, has been identified as a protein that interacts with the cytoplasmic domain of the beta2 subunit of the alphaL/beta2 integrin LFA-1 (2). Patients with leukocyte adhesion molecule (CD11/CD18, beta 2 integrins) deficiency have structural defects in the common beta subunit (CD18), which prevent heterodimer formation and normal cell surface expression of these receptors, leading to life-threatening bacterial infections (3) |
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Western blot - CD11a antibody [EP1285Y]; Anti-CD11a antibody [EP1285Y] at 1/20000 dilution + Jurkat cell lysate at 10 µg/ml.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 129 kDa.Observed band size : 180 kDa .
Immunohistochemistry (Paraffin-embedded sections) - CD11a antibody [EP1285Y]; Immunohistochemical staining of paraffin embedded human spleen tissue using ab52895 at 1/100 dilution.
Immunohistochemistry (Frozen sections) - Anti-CD11a antibody [EP1285Y]; Immunohistochemical analysis of cynomolgus monkey brain tissue, staining CD11a with ab52895 at 1/50 dilution.
Flow Cytometry-CD11a antibody [EP1285Y](ab52895); Overlay histogram showing Jurkat cells stained with ab52895 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.