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Home Antibody All anti-ITGA4 antibodies

Anti-ITGA4 Antibody EPR1355Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300988
  • Rabbit Monoclonal Antibody against ITGA4 (VLA-alpha4) (Clone EPR1355Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400316) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(1)
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Also for ITGA4 (NM_000885)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-terminus of human VLA-alpha4 was used as an immunogen.
Clone NameEPR1355Y IsotypeTissue culture supernatant
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000 - 1:2000; IHC-P: 1:100 - 1:250; ICC/IF: 1:50; IP: 1:40
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor) (ITGA4)
Alternative NameCD49D; IA4
Database LinkNP_000876
Entrez Gene 3676 Human
FunctionVLA-4 is a cell surface heterodimer in the integrin superfamily of adhesion receptors. Anti-VLA-4 antibodies inhibited cytolytic T cell activity, with inhibitory activity directed against the effector T cells rather than their targets. Thus, whereas other VLA receptors appear to mediate cell--matrix interactions, VLA-4 may have a cell--cell adhesion function. Alpha 4 stands apart from all other known integrin alpha subunit sequences because (i) alpha 4 has neither an inserted I-domain, nor a disulfide-linked C-terminal fragment, (ii) its sequence is the most unique and (iii) only alpha 4 has a potential protease cleavage site, near the middle of the coding region, which appears responsible for the characteristic 80,000 and 70,000 Mr fragments of alpha 4 (1). Unlike any other integrin alpha subunit, the intact (150 kDa) alpha 4 subunit of VLA-4 can sometimes be cleaved into two noncovalently associated fragments (80 and 70 kDa) (2). The VLA-4 integrins are indispensable for embryogenesis, haematopoiesis and immune responses, possibly because alpha4 regulates cellular functions differently from other integrins through its cytoplasmic tail (3).
Related PathwayES Cell Differentiation/IPSTransmembraneDruggable Genome Focal adhesionECM-receptor interactionCell adhesion molecules (CAMs)Hematopoietic cell lineageLeukocyte transendothelial migrationRegulation of actin cytoskeletonMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Integrin alpha 4 antibody [EPR1355Y]; Anti-Integrin alpha 4 antibody [EPR1355Y] at 1/2000 dilution + Jurkat cell lysate at 10 ug.Secondary.HRP labelled goat anti rabbit at 1/2000 dilution.Predicted band size : 150 kDa.Observed band size : 150 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Integrin alpha 4 antibody [EPR1355Y]; Immunohistochemical staining of paraffin-embedded human tonsils using 1/100 TA300988

 

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