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Anti-IRS4 Antibody EP907Y
Also for IRS4 (NM_003604)
|A synthetic peptide corresponding to residues on the C-terminus of human IRS-4 was used as an immunogen.|
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, FC
||IHC-P: Use at an assay dependent dilution; WB: 1:5000; IP: 1:20; FC: 1:20 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Does not react with Mouse, Rat
|Homo sapiens insulin receptor substrate 4 (IRS4)|
|IRS4 encodes the insulin receptor substrate 4, a cytoplasmic protein that contains many potential tyrosine and serine/threonine phosphorylation sites. Tyrosine-phosphorylated IRS4 protein has been shown to associate with cytoplasmic signalling molecules that contain SH2 domains. The IRS4 protein is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation.. [provided by RefSeq]. |
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Western blot - IRS4 antibody [EP907Y]; Anti-IRS4 antibody [EP907Y] at 1/5000 dilution + Hek293 cell lysate at 10 ug.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 133 kDa.Observed band size : 133 kDa.
Immunohistochemistry (Paraffin-embedded sections) - IRS4 antibody [EP907Y]; TA303856, at a 1/50 dilution staining Human IRS4 in kidney using Immunohistochemistry, Paraffin embedded tissue.
Flow Cytometry-Anti-IRS4 antibody [EP907Y](TA303856); Overlay histogram showing HEK293 cells stained with TA303856 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.