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Home Antibody All anti-IRS4 antibodies

Anti-IRS4 Antibody EP907Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303856
  • Rabbit monoclonal antibody against IRS-4 (C-term) (EP907Y )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401194) , 20ug
100ul 325 In Stock
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WB(1)
IHC(1)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the C-terminus of human IRS-4 was used as an immunogen.
Clone NameEP907Y IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsIHC-P: Use at an assay dependent dilution; WB: 1:5000; IP: 1:20; FC: 1:20 - 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens insulin receptor substrate 4 (IRS4)
Alternative NameIRS-4; PY160
Database LinkNP_003595
FunctionIRS4 encodes the insulin receptor substrate 4, a cytoplasmic protein that contains many potential tyrosine and serine/threonine phosphorylation sites. Tyrosine-phosphorylated IRS4 protein has been shown to associate with cytoplasmic signalling molecules that contain SH2 domains. The IRS4 protein is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation.. [provided by RefSeq].
Related Pathway
Adipogenesis

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - IRS4 antibody [EP907Y]; Anti-IRS4 antibody [EP907Y] at 1/5000 dilution + Hek293 cell lysate at 10 ug.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 133 kDa.Observed band size : 133 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - IRS4 antibody [EP907Y]; TA303856, at a 1/50 dilution staining Human IRS4 in kidney using Immunohistochemistry, Paraffin embedded tissue.
FC Image
Flow Cytometry-Anti-IRS4 antibody [EP907Y](TA303856); Overlay histogram showing HEK293 cells stained with TA303856 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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