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Anti-IRF7 Antibody EPR4718
Also for IRF7 (NM_001572)
|A synthetic peptide corresponding to residues in human IRF-7 was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, FC
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; FC: 1:100; IHC-Fr: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for ICC or IHC-P.
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Western blot - IRF7 antibody [EPR4718]; All lanes : Anti-IRF7 antibody [EPR4718] at 1/1000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : Raji cell lysate.Lane 3 : HuT78 cell lysate.Lane 4 : RAW264.7 cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 54 kDa.
Immunohistochemistry (Frozen sections) - Anti-IRF7 antibody [EPR4718]; TA307536 was used to detect IRF7 in Human tonsil tissue using Immunohistochemistry (Frozen sections). Human tonsil tissue was frozen and fixed with acetone fixed, sample permeabilized with 0.05% Tween and blocked with 6% BSA for 1 hr at room temp. Primary antibody was diluted 1/100 in 2% BSA in TBS/Tween and incubated for 12 hr at 4Â°C. An Alexa Fluor?488-conjugated Goat anti-rabbit IgG polyclonal(1/25) was used as the secondary antibody. IRF7 staining in green, DAPI in blue.
Flow Cytometry - Anti-IRF7 antibody [EPR4718]; Flow cytometric analysis of permeabilized Jurkat cells using TA307536 (red) or a rabbit IgG (negative) (green).
Flow Cytometry - Anti-IRF7 antibody [EPR4718]; Overlay histogram showing HeLa cells stained with TA307536 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Flow Cytometry - Anti-IRF7 antibody [EPR4718]; Flow cytometry analysis of Plasmacytoid dendritic cells, staining IRF7 with TA307536. .Cells were fixed in paraformaldehyde and fixed in saponin. The sample was incubated with the primary antibody (1/100 in 2% human serum + 0.5 mM EDTA) for 20 minutes at 4Â°C. An AlexaFluor?488-conjugated goat anti-rabbit polyclonal IgG (1/100) was used as the secondary antibody.Gating Strategy: FSC/SSC lymphocytes and then FSC-A/FSC-H singlets