Invalid object name 'antibody_LocusMapping'. Anti-IRF4 Antibody, EP5699 - OriGene
OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-IRF4 antibodies

Anti-IRF4 Antibody EP5699

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310951
  • Rabbit Monoclonal antibody against IRF-4 (IRF4)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419304) , 20ug Explanation
100ul 325 3-7 Days
Add to Shopping Cart
WB(1)
IHC(2)
FC(1)
spacer
Also for IRF4 (NM_002460)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human IRF-4 was used as an immunogen.
Clone NameEP5699 IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:10000 - 1:50000; IHC-P: 1:250 - 1:500; IP: 1:100 - 1:500; FC: 1:100 - 1:1000; ICC/IF: 1:250 - 1:500
BufferPreservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens interferon regulatory factor 4 (IRF4), transcript variant 1
Alternative NameLSIRF; MUM1; NF-EM5
Database LinkNP_002451
FunctionIRF-4 belongs to the IRF (interferon regulatory factor) family of transcription factors, characterized by an unique tryptophan pentad repeat DNA-binding domain. The IRFs are important in the regulation of interferons in response to infection by viruses and the regulation of interferon-inducible proteins.IRF-4 is lymphocyte specific and negatively regulates Toll-like-receptor (TLR) signaling that is central to the activation of innate and adaptive immune systems. A mutation in IRF-4 may be a cause of multiple myeloma (1).
Related Pathway
Apoptosis

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-MUM1 antibody [EP5699]; All lanes : Anti-MUM1 antibody [EP5699] at 1/10000 dilution.Lane 1 : Namalwa cell lysate.Lane 2 : HuT78 cell lysate.Lane 3 : IM9 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 52 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUM1 antibody [EP5699]; Immunohistochemical analysis of paraffin embedded Human lymphoma tissue labelling MUM1, using TA310951 at a dilution of 1/250.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUM1 antibody [EP5699]; Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelling MUM1, using TA310951 at a dilution of 1/250.
FC Image
Flow Cytometry - Anti-MUM1 antibody [EP5699]; Overlay histogram showing Ramos cells stained with TA310951 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title