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Anti-IRF4 Antibody EP5699
Also for IRF4 (NM_002460)
|A synthetic peptide corresponding to residues in human IRF-4 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:10000 - 1:50000; IHC-P: 1:250 - 1:500; IP: 1:100 - 1:500; FC: 1:100 - 1:1000; ICC/IF: 1:250 - 1:500
|Preservative: 0.01% Sodium azide|
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens interferon regulatory factor 4 (IRF4), transcript variant 1|
|LSIRF; MUM1; NF-EM5|
|IRF-4 belongs to the IRF (interferon regulatory factor) family of transcription factors, characterized by an unique tryptophan pentad repeat DNA-binding domain. The IRFs are important in the regulation of interferons in response to infection by viruses and the regulation of interferon-inducible proteins.IRF-4 is lymphocyte specific and negatively regulates Toll-like-receptor (TLR) signaling that is central to the activation of innate and adaptive immune systems. A mutation in IRF-4 may be a cause of multiple myeloma (1).|
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Western blot - Anti-MUM1 antibody [EP5699]; All lanes : Anti-MUM1 antibody [EP5699] at 1/10000 dilution.Lane 1 : Namalwa cell lysate.Lane 2 : HuT78 cell lysate.Lane 3 : IM9 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 52 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUM1 antibody [EP5699]; Immunohistochemical analysis of paraffin embedded Human lymphoma tissue labelling MUM1, using ab133590 at a dilution of 1/250.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUM1 antibody [EP5699]; Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelling MUM1, using ab133590 at a dilution of 1/250.
Flow Cytometry - Anti-MUM1 antibody [EP5699]; Overlay histogram showing Ramos cells stained with ab133590 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.