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Anti-IRF3 TRUEMAB Antibody clone 5D2

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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SKU Description Amount Price Availability*  
TA500465
  • anti-IRF3 mouse monoclonal antibody,clone 5D2
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400600) , 20ug Explanation
100ul $325 In Stock
CF500465
  • Carrier-free (BSA/glycerol-free) IRF3 mouse monoclonal antibody,clone 5D2
100ug $450 3-4 weeks
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WB(2)
IHC(14)

OriGene Data

ImmunogenFull-length protein expressed in 293T cell transfected with human IRF3 expression vector
Clone Nameclone 5D2 IsotypeIgG2b
Species ReactivityHuman, Monkey Concentration1.3 mg/ml
Guaranteed Application *WB, IHC Suggested DilutionsWB 1:2000 IHC 1:50
Predicted MW Explanation 47.2 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens interferon regulatory factor 3 (IRF3), transcript variant 1
Alternative NameMGC94729; interferon regulatory factor 3
Database LinkNP_001562
Entrez Gene 3661 Human
Entrez Gene 718970 Monkey
FunctionIRF3 encodes interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex transl
Related PathwayTranscription FactorsDruggable Genome Toll-like receptor signaling pathwayRIG-I-like receptor signaling pathwayCytosolic DNA-sensing pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF3 (RC209951, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRF3.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-anti-IRF3monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded breast tissue using anti-IRF3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500465, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-IRF3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500465, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500465, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500465, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded lung tissue using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500465, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500465, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500465, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500465, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500465, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded thyroid tissue using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500465, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500465, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500465, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500465, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500465, Dilution 1:50)
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