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Anti-IRF3 Antibody EPR2346
Also for IRF3 (NM_001571)
|A phospho-specific peptide corresponding to residues surrounding Serine 386 of human IRF3 was used as an immunogen. The antibody only detects IRF3 phosphorylated on Serine 386.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; ICC/IF: 1:250 - 1:500
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt,IHC-P or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,IHC-P or IP.
|Homo sapiens interferon regulatory factor 3 (IRF3), transcript variant 1|
|MGC94729; interferon regulatory factor 3|
|A family of interferon (IFN) regulatory factors (IRFs) have been shown to play a role in transcription of IFN genes as well as IFN-stimulated genes. The IRF-3 gene encodes a 50-kDa protein that binds specifically to the IFN-stimulated response element (ISRE) (1). IRF-3 is activated through phosphorylation by the kinases IKK epsilon and/or TBK1. Phosphorylation results in IRF-3 dimerization and removal of an autoinhibitory structure to allow interaction with the coactivators CBP/p300. Phosphorylation at Serine 396 through Serine 405 alleviates autoinhibition to allow interaction with CREB-binding protein and facilitates phosphorylation at Serine 385 or Serine 386 (2). Serine 386 is suggested to be the target of the IRF-3 kinase and critical determinant for the activation of IRF-3 (3). |
Toll-like receptor signaling pathway
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Western blot - IRF3 (phospho S386) antibody [EPR2346]; All lanes : Anti-IRF3 (phospho S386) antibody [EPR2346] at 1/20000 dilution.Lane 1 : Lysate from untreated MCF-7 cells .Lane 2 : Lysate from MCF-7 cells treated with Calyculin A.Lysates/proteins at 10 µg per lane.Secondary.HRP labeled goat anti-rabbit at 1/1000 dilution.Predicted band size : 47 kDa.Observed band size : 50 kDa .
Immunocytochemistry/ Immunofluorescence - IRF3 (phospho S386) antibody [EPR2346]; ab76493 staining IRF3 (green) in untreated MCF7 cells by Immunocytochemistry/ Immunofluorescense. Nuclei were counterstained blue with DAPI.