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Anti-IRF3 Antibody EP2419Y
Also for IRF3 (NM_001571)
|A synthetic peptide corresponding to residues on the C-terminus of human IRF-3 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1: 1000~2000; IHC: 1: 100~250; FC: 1: 10; IP: 1:20
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens interferon regulatory factor 3 (IRF3), transcript variant 1|
|MGC94729; interferon regulatory factor 3|
|A family of interferon (IFN) regulatory factors (IRFs) have been shown to play a role in transcription of IFN genes as well as IFN-stimulated genes. The IRF-3 gene encodes a 50-kDa protein that binds specifically to the IFN-stimulated response element (ISRE) but not to the IRF-1 binding site PRD-I. Overexpression of IRF-3 stimulates expression of the IFN-stimulated gene 15 (ISG15) promoter, an ISRE-containing promoter. he high amino acid homology between IRF-3 and ISG factor 3 gamma polypeptide (ISGF3 gamma) and their similar binding properties indicate that, like ISGF3 gamma, IRF-3 may activate transcription by complex formation with other transcriptional factors, possibly members of the Stat family (1). IRF-3 is expressed constitutively in a variety of tissues, and the relative levels of IRF-3 mRNA do not change in virus-infected or IFN-treated cells. (2). In uninfected cells, the IRF-3 component of DRAF1 resides in the cytoplasm. The cytoplasmic localization of IRF-3 is dependent on a nuclear export signal, and IRF-3 is recognized by the chromosome region maintenance 1 (CRM1) (also known as exportin 1) shuttling receptor. Following infection and specific phosphorylation, IRF-3 accumulates in the nucleus where it associates with CBP and p300 (3). |
Toll-like receptor signaling pathway
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Western blot - IRF3 antibody [EP2419Y] - Carboxyterminal end; All lanes : Anti-IRF3 antibody [EP2419Y] - Carboxyterminal end at 1/2000 dilution.Lane 1 : U937 cell lysate.Lane 2 : Hela cell lysate.Lane 3 : MCF7 cell lysate.Lane 4 : Jurkat cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 47 kDa.Observed band size : 47 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IRF3 antibody [EP2419Y] - Carboxyterminal end; Immunohistochemical analysis of IRF3 in paraffin embedded human tonsils using ab76409 at a 1/100 dilution.
Flow Cytometry - IRF3 antibody [EP2419Y] - Carboxyterminal end; Flow cytometric analysis of permeabilized U937 cells using anti- IRF-3 RabMAb (red) or a rabbit IgG (negative) (green)