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Home Antibody All anti-IRF3 antibodies

Anti-IRF3 Antibody EP2419Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA301106
  • Rabbit Monoclonal Antibody against IRF3 (Clone EP2419Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400600) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(1)
FC(1)
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Also for IRF3 (NM_001571)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the C-terminus of human IRF-3 was used as an immunogen.
Clone NameEP2419Y IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1: 1000~2000; IHC: 1: 100~250; FC: 1: 10; IP: 1:20
Predicted MW Explanation 47 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens interferon regulatory factor 3 (IRF3), transcript variant 1
Alternative NameMGC94729; interferon regulatory factor 3
Database LinkNP_001562
Entrez Gene 3661 Human
FunctionA family of interferon (IFN) regulatory factors (IRFs) have been shown to play a role in transcription of IFN genes as well as IFN-stimulated genes. The IRF-3 gene encodes a 50-kDa protein that binds specifically to the IFN-stimulated response element (ISRE) but not to the IRF-1 binding site PRD-I. Overexpression of IRF-3 stimulates expression of the IFN-stimulated gene 15 (ISG15) promoter, an ISRE-containing promoter. he high amino acid homology between IRF-3 and ISG factor 3 gamma polypeptide (ISGF3 gamma) and their similar binding properties indicate that, like ISGF3 gamma, IRF-3 may activate transcription by complex formation with other transcriptional factors, possibly members of the Stat family (1). IRF-3 is expressed constitutively in a variety of tissues, and the relative levels of IRF-3 mRNA do not change in virus-infected or IFN-treated cells. (2). In uninfected cells, the IRF-3 component of DRAF1 resides in the cytoplasm. The cytoplasmic localization of IRF-3 is dependent on a nuclear export signal, and IRF-3 is recognized by the chromosome region maintenance 1 (CRM1) (also known as exportin 1) shuttling receptor. Following infection and specific phosphorylation, IRF-3 accumulates in the nucleus where it associates with CBP and p300 (3).
Related PathwayTranscription FactorsDruggable Genome Toll-like receptor signaling pathwayRIG-I-like receptor signaling pathwayCytosolic DNA-sensing pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - IRF3 antibody [EP2419Y] - Carboxyterminal end; All lanes : Anti-IRF3 antibody [EP2419Y] - Carboxyterminal end at 1/2000 dilution.Lane 1 : U937 cell lysate.Lane 2 : Hela cell lysate.Lane 3 : MCF7 cell lysate.Lane 4 : Jurkat cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 47 kDa.Observed band size : 47 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IRF3 antibody [EP2419Y] - Carboxyterminal end; Immunohistochemical analysis of IRF3 in paraffin embedded human tonsils using TA301106 at a 1/100 dilution.
FC Image
Flow Cytometry - IRF3 antibody [EP2419Y] - Carboxyterminal end; Flow cytometric analysis of permeabilized U937 cells using anti- IRF-3 RabMAb (red) or a rabbit IgG (negative) (green)

 

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