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Anti-INSR PHOSPHO Antibody EP351(2)Y
Also for INSR (NM_000208)
|A phospho synthetic peptide corresponding to residues surrounding Tyrosine 1185 of human INSR was used as an immunogen. This antibody detects INSR phosphorylated at Tyrosine 1185.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:2000; IP: 1:20; ICC: 1:100 - 1:250
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt or IHC-P.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IHC-P.
|Homo sapiens insulin receptor (INSR), transcript variant 1|
|After removal of the precursor signal peptide, the insulin receptor precursor is post-translationally cleaved into two chains (alpha and beta) that are covalently linked. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. |
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Western blot - Insulin Receptor (phospho Y1185) antibody [EP351(2)Y]; All lanes : Anti-Insulin Receptor (phospho Y1185) antibody [EP351(2)Y] at 1/500 dilution.Lane 1 : HepG2 cell lysate; untreated.Lane 2 : HepG2 cell lysate; Insulin treated.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP conjugated, at 1/2000 dilution.Predicted band size : 156 kDa.Observed band size : 95 kDa .