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Anti-ING4 Antibody

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SKU Description Amount Price Availability*  
TA319198
  • Goat polyclonal anti-p29 ING4 antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC402512) , 20ug Explanation
100ug 325 3-7 Days
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WB(2)

OriGene Data

ImmunogenThis affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding aa 164-175 of Human p29 ING4 protein (Inhibitor of growth family, member 4). This sequence only shows homology to isoform 1 for ING4.
Clone Name Isotype
Species Reactivityhuman Concentration1.28 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:3,000 - 1:12,000, WB: 1:500 - 1:3,000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note p29 ING4 is a tumor suppressor protein similar to ING1 that  may inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation. p29 ING4 has been shown to suppress brain tumor angio-genesis through transcriptional repression of RelA/ NFKB3 target genes when complexed with RelA.  p29 ING4 may also specifically suppress loss of contact inhibition elicited by activated oncogenes such as MYC. p29 ING4 shows a nuclear localization and interacts with EP300, TP53, RelA, inhibits cell growth, and induces apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. Multiple alternatively spliced transcript variants have been observed.  The accession number listed below is for variant (1) that encodes the longest isoform.

Reference Data

Target NameHomo sapiens inhibitor of growth family, member 4 (ING4), transcript variant 9
Alternative Namemy036; p29ING4
Database LinkNP_001121054
Entrez Gene 51147 Human
Function
Related PathwayTranscription FactorsDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Anti-p29 ING4 polyclonal antibody detects ING4 protein by western blot in over expressed cell lysates.  This antibody was used at 1.0 µg/ml to detect ING4 expression in control (-) and transformed U2OS and HeLa cell lysates.  A predominant band corresponding to p29 ING4 is only seen in lysates from transformed cells.  Personnel Communication, Motoko Unoki, NCI, NIH.
WB Image
Anti-p29 ING4 polyclonal antibody detects ING4 protein by western blot.  This antibody was used at 1.0 µg/ml to detect ING4 (lane 2) present in a U2OS whole cell lysate over expressing the protein.  A control lysate (lane 3) shows no background staining. Comparison to MW markers (lane 1) indicates detection of a single band at ~29 kDa corresponding to ING4.   A 4-20% TRIS-glycine gradient gel was used to separate the protein by SDS-PAGE under reducing conditions.  The protein was transferred to nitrocellulose using standard methods.  After blocking using 5% non-fat dry milk in PBS, the membrane was probed with the primary antibody overnight at 4° C followed by washes and reaction with a 1:20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] (code 605-432-013) for 45 min at room temperature.   LICOR's Odyssey® Infrared Imaging System was used to scan and process the image.  Other detection systems will yield similar results.

 

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