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Anti-INCENP Antibody EPR3542(2)
Also for INCENP (NM_001040694)
|A synthetic peptide corresponding to residues in human INCENP was used as an immunogen.|
||Tissue culture supernatant
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000; ICC: 1:100 - 1:250; ICC/IF: Use at an assay dependent concentration
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt,IHC-P or IP.|
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Is unsuitable for Flow Cyt,IHC-P or IP.
|Homo sapiens inner centromere protein antigens 135/155kDa (INCENP), transcript variant 1|
|INCENP, or inner centromere protein, is a chromosomal passenger protein that is a component of the chromosomal passenger complex (CPC), which acts as a key regulator of mitosis (1, 2). The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation, and it is required for chromatin-induced microtubule stabilization and spindle assembly (2). INCENP, Survivin, and Borealin are three components of the CPC complex that regulate the activity and localization of its enzymatic component, the kinase Aurora B (3). INCENP localizes on chromosome arms and inner centromeres from prophase through metaphase, subsequently transferring to the spindle midzone and midbody from anaphase through cytokinesis (2). During telophase, INCENP is located within the midbody in the intercellular bridge, where it is discarded after cytokinesis (1).|
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Western blot - Anti-INCENP antibody [EPR3542(2)]; All lanes : Anti-INCENP antibody [EPR3542(2)] at 1/1000 dilution.Lane 1 : Molt4 cell lysate.Lane 2 : Jurkat cell lysate.Lane 3 : PC3 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution.Predicted band size : 105 kDa.Observed band size : 140 kDa .
Immunocytochemistry/ Immunofluorescence - Anti-INCENP antibody [EPR3542(2)]; Immunofluorescent staining of HeLa cells labelling INCENP with TA307618 at 1/100.