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Anti-INA Antibody EP676Y
|A synthetic peptide corresponding to residues in the tail domain of human NF-66 was used as immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||FC: 1:25, ICC: 1:250-500, IHC: 1:250-500, IP: 1:50, WB: 1:10,000-50,000,
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Apteronotus leptorhynchus
|Homo sapiens internexin neuronal intermediate filament protein, alpha (INA)|
|NEF5; NF-66; TXBP-1|
|Neurofilaments (NF) are cytoplasmic intermediate filaments (IFs) found in neurons. NF-66, also known as a-internexin, is a member of the IF family. Classified as a type IV IF, NF-66 is expressed by most neurons and has been shown to co-assemble with other NF triple proteins (light, medium and heavy NF) (1-2). In the adult brain, NF-66 is expressed at relatively low levels compared to the other NF proteins (3). NF-66 as also been shown to assemble into 10mm diameter IF in vitro. |
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Western blot - alpha Internexin antibody [EP676Y]; Anti-alpha Internexin antibody [EP676Y] at 1/10000 dilution + SHSY5Y cell lysate at 10 µg.Predicted band size : 66 kDa.Observed band size : 60 kDa .
Immunohistochemistry (Paraffin-embedded sections) - alpha Internexin antibody [EP676Y]; Positive staining of alpha Internexin on paraffin-embedded human brain tissue (A) using ab40758 diluited 1:250. Negative staining of paraffin-embedded human colon (B), Kidney (C) and uterus (D) tissues using ab40758 under the same conditions.
Immunofluorescence - alpha Internexin antibody [EP676Y]; Immunofluorescent staining of alpha Internexin on PC 12 cells using ab40758 diluted 1:250.