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Anti-ILF3 Antibody EPR3626
Also for ILF3 (NM_012218)
|A synthetic peptide corresponding to residues in human ILF3 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IP, FC
||FC: 1:50, ICC: 1:250 - 500, IHC: 1:100 - 250, IP: 1:50, WB: 1:10,000 - 100,000,
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Does not react with Rat
|Homo sapiens interleukin enhancer binding factor 3, 90kDa (ILF3), transcript variant 1|
|CBTF; DRBF; DRBP76; MMP4; MPHOSPH4; MPP4; NF-AT-90; NF110; NF110b; NF90; NF90a; NF90b; NFAR; NFAR-1;|
|Interleukin enhancer-binding factor 3 (ILF3) is a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein was first discovered to be a subunit of the nuclear factor of activated T-cells (NFAT); a transcription factor required for T-cell expression of interleukin 2 (1). ILF3 regulates protein arginine N-methyltransferase 1 activity and has been suggested to regulate transcription of the IL2 gene during T-cell activation. ILF3 promotes the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA (2). |
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Western blot - ILF3 antibody [EPR3626]; All lanes : Anti-ILF3 antibody [EPR3626] at 1/100000 dilution.Lane 1 : Raji cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : K562 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit antibody at 1/2000 dilution.Predicted band size : 95 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ILF3 antibody [EPR3626]; TA307123 at 1/100 dilution staining ILF3 in paraffin-embedded Human kidney tissue, by immunohistochemistry.
Immunoprecipitation - Anti-ILF3 antibody [EPR3626]; ILF3 was immunoprecipitated using 0.5mg K562 whole cell extract, 5ug of Rabbit monoclonal to ILF3 and 50ul of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, K562 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70Â°C; 10ul of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with TA307123.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) .Band: 95kDa; ILF3
Flow Cytometry-Anti-ILF3 antibody [EPR3626](TA307123); Overlay histogram showing HeLa cells stained with TA307123 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22Â°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22Â°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (1Âµg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.