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Home Antibody All anti-ILF3 antibodies

Anti-ILF3 Antibody EPR3626

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307123
  • Rabbit monoclonal antibody against ILF3 (clone EPR3626 )
  • FREE positive control: HEK293T cell transient overexpression lysate (LC415895) , 20ug
100ul 325 3-7 Days
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WB(1)
IP(1)
IHC(1)
FC(1)
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Also for ILF3 (NM_012218)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human ILF3 was used as an immunogen.
Clone NameEPR3626 IsotypeIgG
Species ReactivityHuman, Mouse Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IP, FC Suggested DilutionsFC: 1:50, ICC: 1:250 - 500, IHC: 1:100 - 250, IP: 1:50, WB: 1:10,000 - 100,000,
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Rat

Reference Data

Target NameHomo sapiens interleukin enhancer binding factor 3, 90kDa (ILF3), transcript variant 1
Alternative NameCBTF; DRBF; DRBP76; MMP4; MPHOSPH4; MPP4; NF-AT-90; NF110; NF110b; NF90; NF90a; NF90b; NFAR; NFAR-1;
Database LinkNP_036350
FunctionInterleukin enhancer-binding factor 3 (ILF3) is a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein was first discovered to be a subunit of the nuclear factor of activated T-cells (NFAT); a transcription factor required for T-cell expression of interleukin 2 (1). ILF3 regulates protein arginine N-methyltransferase 1 activity and has been suggested to regulate transcription of the IL2 gene during T-cell activation. ILF3 promotes the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA (2).
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - ILF3 antibody [EPR3626]; All lanes : Anti-ILF3 antibody [EPR3626] at 1/100000 dilution.Lane 1 : Raji cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : K562 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit antibody at 1/2000 dilution.Predicted band size : 95 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ILF3 antibody [EPR3626]; TA307123 at 1/100 dilution staining ILF3 in paraffin-embedded Human kidney tissue, by immunohistochemistry.
IP Image
Immunoprecipitation - Anti-ILF3 antibody [EPR3626]; ILF3 was immunoprecipitated using 0.5mg K562 whole cell extract, 5ug of Rabbit monoclonal to ILF3 and 50ul of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, K562 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70°C; 10ul of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with TA307123.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) .Band: 95kDa; ILF3
FC Image
Flow Cytometry-Anti-ILF3 antibody [EPR3626](TA307123); Overlay histogram showing HeLa cells stained with TA307123 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

 

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