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Home Antibody All anti-IL7 antibodies

Anti-IL7 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319317
  • Rabbit polyclonal IL-7 antibody Biotin Conjugated
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC424471) , 20ug
100ug 325 3-7 Days
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WB(2)
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Also for IL7 (NM_000880)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenThis purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-7 protein.
Clone Name IsotypeIgG
Species Reactivityhuman ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB Suggested DilutionsELISA: 1:20,000-1:100,000, WB: 1:2,000-1:10,000, IHC: 1:1,000-1:5,000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note Interleukin 7 (IL7) is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA). IL7 stimulates the proliferation of CD4+/CD8+ cells. The proliferative response of thymocytes to IL7 is not affected by antibodies to the T cell growth factors such as IL2, IL4 and IL6, suggesting that IL7 is capable of stimulating T cell proliferation through a pathway independent of the known T cell growth factors. Mature T cells respond to IL7 and Con A, but not to IL7 alone. In myeloid lineage cells, IL7 upregulates the production of pro-inflammatory cytokines and stimulates the tumoricidal activity of monocytes/macrophages. IL7 is expressed by adherent stromal cells from various tissues.

Reference Data

Target NameHomo sapiens interleukin 7 (IL7), transcript variant 1
Alternative NameIL-7
Database LinkNP_000871
Function
Related Pathway
Cytokine-cytokine receptor interaction
Hematopoietic cell lineage
Jak-STAT signaling pathway

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WB Image
Western Blot showing detection of Human IL-7. 50ng of Human IL-7 (Lane 1) was run on a 4-20% gel and transferred to 0.45 µm nitrocellulose. After blocking with 5% Blotto (p/n B501-0500) 30 min at 20°C, Anti-Human IL-7 (RABBIT) Antibody Biotin Conjugated (p/n TA319317) secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070). HRP Streptavidin (p/n S000-03) was used at 1:40,000 in MB-070 for 30 min at 20°C and imaged using the Bio-Rad VersaDoc® 4000 MP. Arrow indicates correct 17 kDa molecular weight position expected for Human IL-7.
WB Image
anti-Human IL-7 antibody shows detection of a band ~17 kDa in size corresponding to recombinant human IL-7. The identity of the faint higher molecular weight band may represent a homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000 in blocking buffer (p/n MB-070) for 30 min at RT followed by reaction with FemtoMax™ chemiluminescent substrate. Image was captured using VersaDoc™ MP 4000 imaging system (Bio-Rad).

 

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