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|This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-7 protein.|
||Lot dependent; please refer to CoA along with shipment
||ELISA: 1:20,000-1:100,000, WB: 1:2,000-1:10,000, IHC: 1:1,000-1:5,000
|0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Interleukin 7 (IL7) is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA). IL7 stimulates the proliferation of CD4+/CD8+ cells. The proliferative response of thymocytes to IL7 is not affected by antibodies to the T cell growth factors such as IL2, IL4 and IL6, suggesting that IL7 is capable of stimulating T cell proliferation through a pathway independent of the known T cell growth factors. Mature T cells respond to IL7 and Con A, but not to IL7 alone. In myeloid lineage cells, IL7 upregulates the production of pro-inflammatory cytokines and stimulates the tumoricidal activity of monocytes/macrophages. IL7 is expressed by adherent stromal cells from various tissues.
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WB showing detection of Human IL-7. 50ng of Human IL-7 (Lane 1) was run on a 4-20% gel and transferred to 0.45µm nitrocellulose. After blocking with 5% Blotto (p/n B501-0500) 30 min at 20°C, Anti-Human IL-7 (RABBIT) Antibody Biotin Conjugated (p/n TA319317) secondary antibody was used at 1:1000. HRP Streptavidin (p/n S000-03) was used at 1:40,000. Arrow indicates correct 17 kDa molecular weight position expected for Human IL-7.
anti-Human IL-7 antibody shows detection of a band ~17 kDa in size corresponding to recombinant human IL-7. The identity of the faint higher molecular weight band may represent a homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000.