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Home Antibody All anti-IL6 antibodies

Anti-IL6 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319282
  • Rabbit polyclonal anti-IL-6 antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC424617) , 20ug Explanation
1mL 325 3-7 Days
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WB(1)
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Also for IL6 (NM_000600)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenThis whole rabbit serum was prepared by repeated immunizations with recombinant human IL-6 produced in E.coli.
Clone Name IsotypeIgG
Species Reactivityhuman Concentration74 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,000, IHC: 1:400 - 1:800, IP: 1:400 - 1:800
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note Anti IL-6 Antibody recognizes IL-6 that is a secreted cytokine with a wide variety of biological functions. IL-6 is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. IL-6 induces myeloma and plasmacytoma growth and induces nerve cells differentiation and acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. IL-6 also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. Anti-IL-6 antibody is ideal for investigators involved in Immunology and Cancer research

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot using anti-IL6 antibody. Protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. The blot shows detection of a band ~21 kDa in size corresponding to anti-IL6 antibody. Molecular weight markers are also shown (MW). After transfer, the membrane was blocked for 30 minutes with 1% BSA-TBST. Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000 in blocking buffer (p/n MB-070) for 30 min at RT followed by reaction with FemtoMax™ chemiluminescent substrate. Image was captured using VersaDoc™ MP 4000 imaging system (Bio-Rad).

 

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