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Anti-IL27 Antibody 3H12.F10


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SKU Description Amount Price Availability*  
  • Rat monoclonal Mouse IL-27/P28 antibody
100ug $325 3-7 Days
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OriGene Data

ImmunogenThis Protein A purified monoclonal antibody was produced in rats by repeated immunizations with mature length recombinant mouse p28 protein (produced in E.coli) followed by hybridoma development.
Clone Name3H12.F10 Isotype
Species ReactivityMouse Concentration1.0 mg/mL
Guaranteed Application *WB, FC Suggested DilutionsELISA: 1:10,000, WB: 1:1000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note Mouse IL-27/p28 Subunit, also known as Interleukin-30, is a member of the IL-12 family of cytokines. When combined with EBI3 (Epstein-Barr virus induced gene 3), the heterodimer formed is IL-27. Mouse p28 is a proinflammatory cytokine inducing immunomodulatory effects. Current research is underway to delineate specific biological functions.

Reference Data

Target NameMus musculus interleukin 27 (Il27)
Alternative NameIL-27; IL-27p28; Il30; p28
Database LinkNP_663611
Entrez Gene 246779 Mouse
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Anti-IL-27/p28 antibody in western blot shows detection of recombinant mouse IL-27/p28. Recombinant protein (0.1 µg) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The expected band is approximately 26 kDa in size. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-IL-27/p28 antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1:40,000 in blocking buffer (p/n MB-070) for 30 min at RT. Data was collected using Bio-Rad VersaDoc® 4000 MP imaging system.
Mouse peritoneal macrophages were grown in culture for 24 hours, stimulated with 10ng/mL IFN? and 1ug/mL LPS for 14 hours and incubated for 4 hours with Bredfeldin A. Cells were harvested, washed, aliquoted 1x106 cells per sample, and fixed and permeabilized according to a standard protocol. Samples were stained with biotinylated primary anti-mouse p28 antibody at (0.1 - 10ug/mL primary antibody alongside negative controls of unstimulated cells and isotype controls. Cells were stained with 0.25ug/mL rat anti-mouse CD107b conjugated Alexa Fluor 647 and PHYCOERYTHRIN Conjugated secondary at 1:100 and analyzed by flow cytometry. Stimulated cells showed increase PE staining (horizontal axis) when compared with unstimulated cells.


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