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Home Antibody All anti-IFT57 antibodies

Anti-IFT57 TRUEMAB Antibody Clone OTI4F6

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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SKU Description Amount Price Availability*  
TA500918 anti-IFT57 (HIPPI) mouse monoclonal antibody, clone OTI4F6 (formerly 4F6) 100ul $325 In Stock
LC402637 IFT57 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500918 Carrier-free (BSA/glycerol-free) IFT57 mouse monoclonal antibody, clone OTI4F6 (formerly 4F6) 100ug $450 3-4 weeks
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WB(2)
IHC(4)
IF(1)
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Also for IFT57 (NM_018010)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human IFT57(NP_060480) produced in HEK293T cell.
Clone NameClone OTI4F6 IsotypeIgG1
Species ReactivityHuman Concentration0.8 mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB 1:2000, IHC 1:50, IF 1:100,
Predicted MW Explanation 49.1 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens intraflagellar transport 57 (IFT57)
Alternative NameESRRBL1; HIPPI; MHS4R2
Database LinkNP_060480
Entrez Gene 55081 Human
FunctionRequired for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway (By similarity). Has pro-apoptotic function via its interaction with HIP1,leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3'present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear
Related PathwayDruggable Genome Huntington's disease

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IFT57 (RC204116, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IFT57.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IFT57 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
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Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-IFT57 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500918, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-IFT57mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500918, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-IFT57mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500918, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-IFT57mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500918, Dilution 1:50)
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Anti-IFT57 mouse monoclonal antibody (TA500918) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IFT57(RC204116).
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