 |
|
|
|
Home All anti-IDH1 antibodies
Anti-IDH1 TrueMAB Antibody Clone 1D1
TrueMAB™ Antibodies - Made against Authentic Protein Antigens
* Buy one primary antibody, get 50% off a GAPDH loading control antibody.
Also for IDH1 (NM_005896)
OriGene Data
| Synthetic peptide around the R132 region of the human IDH conjugated to KLH |
| Clone 1D1 |
IgG1 |
| Human , Dog , Rat , Monkey , Mouse |
0.5~1.0 mg/ml (Lot Dependent) |
| WB, IHC |
WB 1:500, IHC 1:150, |
| PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
| Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose) |
Reference Data
| Homo sapiens isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) |
| IDCD; IDH; IDP; IDPC; PICD |
| NP_005887 |
| Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [provided by RefSeq, Jul 2008]. |
|
* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.
WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDH1 (RC210582, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDH1.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IDH1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
IHC Image
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-IDH1 mouse monoclonal antibody. (TA800427)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-IDH1 mouse monoclonal antibody. (TA800427)
IHC Image
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-IDH1 mouse monoclonal antibody. (TA800427)
Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:
- HRP
- Biotin
- PE
- FITC
- DyLight® 488
- DyLight® 550
- DyLight® 594
- DyLight® 633
* DyLight is a trademark of Thermo-Fisher Scientific
Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.
Contact us with your conjugation/bulk request: antibody@origene.com or 888-267-4436 (Tech Support)
|
 |
