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Home Antibody All anti-IDH1 antibodies

Anti-IDH1 TRUEMAB Antibody Clone 3G9

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA800406
  • IDH1 (Isocitrate Dehydrogenase 1) mouse monoclonal antibody, Clone 3G9
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401782) , 20ug Explanation
100ul 325 In Stock
CF800406
  • Carrier-free (BSA/glycerol-free) IDH1 mouse monoclonal antibody,clone 3G9
100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(3)
IHC(2)
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Also for IDH1 (NM_005896)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenSynthetic peptide around the R132H mutation region of the human IDH conjugated to KLH
Clone NameClone 3G9 IsotypeIgG1
Species ReactivityHuman , Dog , Rat , Mouse Concentration1 mg/ml
Guaranteed Application *WB, IHC Suggested Dilutions WB 1:2000, IHC 1:150,
Predicted MW Explanation 46.5 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens isocitrate dehydrogenase 1 (NADP+), soluble (IDH1), transcript variant 1
Alternative NameHEL-216; HEL-S-26; IDCD; IDH; IDP; IDPC; PICD
Database LinkNP_005887
Entrez Gene 3417 Human
Entrez Gene 15926 Mouse
Entrez Gene 24479 Rat
Entrez Gene 478889 Dog
FunctionIsocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [provided by RefSeq, Jul 2008].
Related Pathway Citrate cycle (TCA cycle)Glutathione metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDH1 (RC210582, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDH1.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IDH1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
WB Image
HEK293T cells were either not tranfected (left lane "293T") or transfected with pCMV6-ENTRY IDH1 (wild type-SKU# RC210582, middle lane "WT") or pCMV6-ENTRY IDH1 mutated (R132H mutation-SKU# RC400096, right lane "R132H") cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (10 ug per lane) were separated by SDS-PAGE and immunoblotted with TA800406 (1:500) and then goat anti-mouse IgG-HRP (1:2000).
IHC Image
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-IDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800406)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-IDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800406)

 

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