Home Antibody All anti-HUWE1 antibodies
Also for HUWE1 (NM_031407)
|ARF-BP1 antibody was raised against a 19 amino acid peptide from near the carboxy terminus of human ARF-BP1.|
|Human, Mouse, Rat
||ELISA, WB: 1 ug/ml, ICC: 5 ug/ml, IF: 20 ug/ml
|PBS containing 0.02% sodium azide.|
|Affinity chromatography purified via peptide column
|Homo sapiens HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1)|
|ARF-BP1; HECTH9; HSPC272; Ib772; LASU1; MULE; URE-B1; UREB1|
Entrez Gene 10075 Human
Entrez Gene 59026 Mouse
Entrez Gene 501546 Rat
|The ARF tumor suppressor is a critical regulator of p53 stability. In addition to p53, ARF1 binds to other proteins such as MDM2 and ARF-BP1, a large protein containing HECT, UBA and WWE motifs. ARF-BP1 directly binds and ubiquitinates p53; this activity is inhibited by ARF, indicating that ARF-BP1 is a critical mediator of the p53-dependent and p53-independent tumor suppressor functions of ARF. ARF-BP1 can also catalyze the polyubiquitination of Mcl-1, an anti-apoptotic Bcl-2 family member involved in DNA damage-induced apoptosis. Elimination of ARF-BP1 expression by RNA interference stabilized Mcl-1 protein, resulting in an attenuation of apoptosis induced by DNA-damage agents.|
|Druggable Genome Ubiquitin mediated proteolysis|
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Western blot analysis of ARF-BP1 in Daudi cell lysate with ARF-BP1 antibody at 1 ug/mL.
Immunocytochemistry of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 5 ug/mL.