OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-HUS1 antibodies

Anti-HUS1 Antibody EPR5132

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307868
  • Rabbit monoclonal antibody against HUS1(clone EPR5132)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC401435) , 20ug
100ul $325 3-7 Days
Add to Shopping Cart
WB(1)
IHC(1)
FC(1)
spacer
Also for HUS1 (NM_004507)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human HUS1 was used as an immunogen.
Clone NameEPR5132 IsotypeTissue culture supernatant
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; FC: 1:100 - 1:500
BufferDoes not react with Mouse, Rat. Is unsuitable for ICC.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for ICC.

Reference Data

Target NameHomo sapiens HUS1 checkpoint homolog (S. pombe) (HUS1), transcript variant 1
Alternative NamehHUS1
Database LinkNP_004498
FunctionHUS1 is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. It forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and it is thought to be an early checkpoint signaling event.
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - HUS1 antibody [EPR5132]; All lanes : Anti-HUS1 antibody [EPR5132] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : K562 cell lysate.Lane 3 : A431 cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 32 kDa.Observed band size : 34 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HUS1 antibody [EPR5132]; Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue using TA307868 at a dilution of 1/100.
FC Image
Flow Cytometry - Anti-HUS1 antibody [EPR5132]; Overlay histogram showing HeLa cells stained with TA307868 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title