Home Antibody All anti-HSPD1 antibodies
Also for HSPD1 (NM_002156)
|A synthetic peptide corresponding to residues in human Heat Shock Protein 60 was used as immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||IHC-Fr: Use at an assay dependent concentration; WB: 1:20000 - 1:50000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:250 - 1:500; FC: 1:20; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified (Protein A or G Sepharose)
|Homo sapiens heat shock 60kDa protein 1 (chaperonin) (HSPD1), transcript variant 1|
|CPN60; GROEL; HLD4; HSP-60; HSP60; HSP65; HuCHA60; SPG13|
|The 60 kDa heat shock protein (HSP60) is a highly conserved protein that act as a chaperone involved in correct folding of newly synthesized proteins (1-2). Mainly expressed in mitochondria, HPS60 has also been found in the cytosol of normal heart and muscle cells. In normal cardiac myocytes, cytosolic HSP60 complexes with Bax, Bak and Bcl-XL (3). While over expression of HSP60 prevents apoptosis in cardiac cells; is has also been shown that HSP60 accelerated the activation of Caspase 3 during apoptosis (4-5). Therefore, the role of HSP60 in apoptosis may be both pro- and anti-apoptotic, depending on the cell type, or apoptotic stimulus.|
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Western blot - Hsp60 antibody; Anti-Hsp60 antibody at 1/50000 dilution + T47D cell lysate at 10 µg.Secondary.Goat anti -rabbit HRP at 1/2000 dilution.Predicted band size : 60 kDa.Observed band size : 60 kDa.
Immunohistochemistry (Paraffin-embedded sections) - Hsp60 antibody; Immunohistochemical analysis of paraffin-embedded human breast adenocarcinoma using ab46798 at 1/250 dilution
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Hsp60 antibody; ab46798 staining Hsp60 from human colon tissue by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were formaldehyde fixed prior to blocking in 10% serum for 2 hours at 21°C. The primary antibody was diluted 1/500 and incubated with the sample for 2 hours at 21°C. Alexa fluor? 594 goat polyclonal, diluted 1/5000, was used as the secondary.
Immunocytochemistry/ Immunofluorescence-Hsp60 antibody(ab46798); ICC/IF image of ab46798 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43?M.