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Home Antibody All anti-HSPD1 antibodies

Anti-HSPD1 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300644
  • Rabbit Polyclonal Antibody against HSPD1
  • FREE positive control: HEK293T cell transient overexpression lysate (LC400783) , 20ug
100ul $379 In Stock
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WB(1)
IHC(2)
IF(1)
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Also for HSPD1 (NM_002156)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Heat Shock Protein 60 was used as immunogen.
Clone Name IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF Suggested DilutionsIHC-Fr: Use at an assay dependent concentration; WB: 1:20000 - 1:50000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:250 - 1:500; FC: 1:20; IP: 1:50
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Protein A purified

Reference Data

Target NameHomo sapiens heat shock 60kDa protein 1 (chaperonin) (HSPD1), transcript variant 1
Alternative NameCPN60; GROEL; HLD4; HSP-60; HSP60; HSP65; HuCHA60; SPG13
Database LinkNP_002147
FunctionThe 60 kDa heat shock protein (HSP60) is a highly conserved protein that act as a chaperone involved in correct folding of newly synthesized proteins (1-2). Mainly expressed in mitochondria, HPS60 has also been found in the cytosol of normal heart and muscle cells. In normal cardiac myocytes, cytosolic HSP60 complexes with Bax, Bak and Bcl-XL (3). While over expression of HSP60 prevents apoptosis in cardiac cells; is has also been shown that HSP60 accelerated the activation of Caspase 3 during apoptosis (4-5). Therefore, the role of HSP60 in apoptosis may be both pro- and anti-apoptotic, depending on the cell type, or apoptotic stimulus.
Related Pathway

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WB Image
Western blot - Hsp60 antibody; Anti-Hsp60 antibody at 1/50000 dilution + T47D cell lysate at 10 µg.Secondary.Goat anti -rabbit HRP at 1/2000 dilution.Predicted band size : 60 kDa.Observed band size : 60 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Hsp60 antibody; Immunohistochemical analysis of paraffin-embedded human breast adenocarcinoma using TA300644 at 1/250 dilution
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Hsp60 antibody; TA300644 staining Hsp60 from human colon tissue by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were formaldehyde fixed prior to blocking in 10% serum for 2 hours at 21°C. The primary antibody was diluted 1/500 and incubated with the sample for 2 hours at 21°C. Alexa fluor? 594 goat polyclonal, diluted 1/5000, was used as the secondary.
IF Image
Immunocytochemistry/ Immunofluorescence-Hsp60 antibody(TA300644); ICC/IF image of TA300644 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43?M.

 

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