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Anti-HSPB1 Antibody EP2293Y
Also for HSPB1 (NM_001540)
|A synthetic phospho-peptide corresponding to residues surrounding Serine 15 of human HSP27 was used as immunogen. The antibody will detect HSP27 phosphorylation on Serine 15.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:2000 - 1:5000; ICC: 1:100 - 1:250
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt,IHC-P or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,IHC-P or IP.
|Homo sapiens heat shock 27kDa protein 1 (HSPB1)|
|CMT2F; HMN2B; HS.76067; Hsp25; HSP27; HSP28; SRP27|
|Heat shock protein (HSP) 27 is one of the small HSPs, and is involved in stress resistance and actin organization. HSP27 is expressed in response to environmental stresses such as heat shock, or estrogen stimulation in MCF-7 cells (1,2). HSP27 is phosphorylated at serines 15, 78 and 82 by MAPKAP kinase 2 as a result of the activation of the p38 MAP kinase pathway (3,4). |
MAPK signaling pathway
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Western blot - Hsp27 ( S15) antibody [EP2293Y]; All lanes : Anti-Hsp27 (phospho S15) antibody [EP2293Y] at 1/5000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : HeLa cell lysate, heat treated at 44°C.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit-HRP at 1/1000 dilution.developed using the ECL technique.Predicted band size : 23 kDa.Observed band size : 27 kDa .
Immunocytochemistry/ Immunofluorescence - Hsp27 ( S15) antibody [EP2293Y]; HeLa cells stained with ab76313 at 1/100 dilution.