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Home Antibody All anti-HSPB1 antibodies

Anti-HSPB1 Antibody EP1724

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA301049
  • Rabbit Monoclonal Antibody against HSPB1 (Clone EP1724Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400587) , 20ug Explanation
100ul $325 3-7 Days
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WB(1)
IHC(1)
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Also for HSPB1 (NM_001540)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-terminus of human Hsp 27 was used as an immunogen.
Clone NameEP1724 IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:500 - 1:2000; IHC-P: 1:100 - 1:250; ICC: 1:50 - 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens heat shock 27kDa protein 1 (HSPB1)
Alternative NameCMT2F; HEL-S-102; HMN2B; HS.76067; Hsp25; HSP27; HSP28; SRP27
Database LinkNP_001531
Entrez Gene 3315 Human
FunctionHeat shock protein (HSP) 27 is one of the small HSPs, and is involved in stress resistance and actin organization. HSP27 is expressed in response to environmental stresses such as heat shock, or estrogen stimulation in MCF-7 cells (1,2). HSP27 is phosphorylated at serines 15, 78 and 82 by MAPKAP kinase 2 as a result of the activation of the p38 MAP kinase pathway (3,4).
Related Pathway MAPK signaling pathwayVEGF signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Hsp27 antibody [EP1724Y] - Aminoterminal end; Anti-Hsp27 antibody [EP1724] at 1/500 dilution + HeLa cell lysate at 10 ug.Secondary.goat anti rabbit, HRP labeled, at 1/2000 dilution.Predicted band size : 23 kDa.Observed band size : 23 kDa.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Hsp27 antibody [EP1724Y] - Aminoterminal end; Immunohistochemical analysis of Hsp27 expression in paraffin embedded human cervical carcinoma (left) and human breast carcinoma (right) using 1/100 TA301049
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