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Home Antibody All anti-HSPA9 antibodies

Anti-HSPA9 TRUEMAB Antibody Clone OTI9F8

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500516 HSPA9 mouse monoclonal antibody, clone OTI9F8 (formerly 9F8) 100ul $379 In Stock
LC401334 HSPA9 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500516 Carrier-free (BSA/glycerol-free) HSPA9 mouse monoclonal antibody, clone OTI9F8 (formerly 9F8) 100ug $450 3-4 weeks
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WB(2)
IHC(6)
IF(2)
FC(2)
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Also for HSPA9 (NM_004134)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human HSPA9 (NP_004125) produced in HEK293T cell.
Clone NameClone OTI9F8 IsotypeIgG2b
Species ReactivityHuman , Dog , Rat , Monkey Concentration1 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:1000~2000, IHC 1:50, IF 1:50, FLOW 1:100,
Predicted MW Explanation 73.5 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens heat shock 70kDa protein 9 (mortalin) (HSPA9)
Alternative NameCSA; GRP-75; GRP75; HEL-S-124m; HSPA9B; MOT; MOT2; MTHSP75; PBP74
Database LinkNP_004125
Entrez Gene 3313 Human
Entrez Gene 291671 Rat
Entrez Gene 714506 Monkey
Entrez Gene 474697 Dog
FunctionThis gene encodes a member of the heat shock protein 70 gene family. The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. A pseudogene of this gene is found on chromosome 2.
Related PathwayStem cell - Pluripotency RNA degradation

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA9 (RC201397, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA9.
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Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-HSPA9 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-HSPA9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500516)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-HSPA9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500516)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HSPA9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500516)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-HSPA9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500516)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-HSPA9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500516)
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-HSPA9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500516)
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Anti-HSPA9 mouse monoclonal antibody (TA500516) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HSPA9(RC201397).
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Immunofluorescent staining of HepG2 cells using anti-HSPA9 mouse monoclonal antibody (TA500516).
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Flow cytometric Analysis of Hela cells, using anti-HSPA9 antibody(TA500516),(Red), compared to a nonspecific negative control antibody,(Blue).
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Flow cytometric Analysis of Jurkat cells, using anti-HSPA9 antibody(TA500516),(Red), compared to a nonspecific negative control antibody,(Blue).
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