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Anti-HSPA8 Antibody EP1531Y
Also for HSPA8 (NM_006597)
|A synthetic peptide corresponding to residues near the C-terminus of human Hsp73 was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||FC: 1:30; IP: 1:10; WB: 1:500; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens heat shock 70kDa protein 8 (HSPA8), transcript variant 1|
|HEL-33; HEL-S-72p; HSC54; HSC70; HSC71; HSP71; HSP73; HSPA10; LAP-1; LAP1; NIP71|
Entrez Gene 3312 Human
Entrez Gene 15481 Mouse
Entrez Gene 24468 Rat
|Hsp73 is a normal cell constituent which is synthesized in increased abundance under adverse environmental circumstances, such as non-physiological temperatures or treatment with amino acid analogues (1). Data has shown a molecular mechanism for cytokine-mediated posttranscriptional regulation of Bim mRNA by Hsc70, which binds to AU-rich elements (AREs) in the 3'-untranslated region of specific mRNAs and enhances their stability. The RNA binding potential of Hsc70 is regulated by cochaperones including Bag-4 (also SODD), CHIP, Hip, and Hsp40 (2). Hsp73 protein expression was detected in the cytoplasm of melanoma cells in primary tumors and metastases. In primary melanomas, the proportion of Hsp73 protein expressing cells correlated with tumor thickness which was found to be statistically significant (3). |
|Stem cell - Pluripotency SpliceosomeMAPK signaling pathwayEndocytosisAntigen processing and presentation|
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Western blot - Hsc70 antibody [EP1531Y]; Anti-Hsc70 antibody [EP1531Y] at 1/500 dilution + HeLa cell lysate at 10 ug.Secondary.Goat anti-Rabbit HRP labeled at 1/2000 dilution.Predicted band size : 71 kDa.Observed band size : 71 kDa.
Immunohistochemistry (Paraffin-embedded sections) - Hsc70 antibody [EP1531Y]; Ab51052 (1/250) staining Hsc70 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Hsc70 antibody [EP1531Y]; Immunofluorescent staining of HeLa cells using TA300865 (1/100).