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Anti-HSPA1B Antibody EP1007Y


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SKU Description Amount Price Availability*  
TA303852 Rabbit monoclonal antibody against Hsp70 (C-term)(EP1007Y) 100ul $325 In Stock
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-term of human Hsp70 was used as an immunogen.
Clone NameEP1007Y IsotypeIgG
Species ReactivityRat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsFC: 1:100 - 1:1000; IP: 1:50; WB: 1:20000; IHC-P: Use at an assay dependent concentration
Predicted MW Explanation 85kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for ICC/IF.

Reference Data

Target NameHomo sapiens heat shock 70kDa protein 1B (HSPA1B)
Alternative NameHSP70-1B; HSP70-2
Database LinkNP_005337
Entrez Gene 3304 Human
Entrez Gene 294254 Rat
FunctionThis intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq].
Related Pathway SpliceosomeMAPK signaling pathwayEndocytosisAntigen processing and presentation

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Hsp70 antibody [EP1007Y]; Anti-Hsp70 antibody [EP1007Y] at 1/20000 dilution + HeLa cell lysate at 10 ug.Secondary.Goat anti-rabbit HRP labelled, at 1/2000 dilution.Observed band size : 70 kDa .

WB Image
Western blot - Anti-Hsp70 antibody [EP1007Y]; .Exposure time : 2 minutes

IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Hsp70 antibody [EP1007Y]; Ab45133 at a 1/100 dilution staining human Hsp70 in human prostate carcinoma by immunohistochemistry using paraffin embedded tissue.

FC Image
Flow Cytometry - Anti-Hsp70 antibody; Overlay histogram showing Jurkat cells stained with TA303852 (red line). The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1:2000. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. This antibody gave a positive signal in Jurkat cells under the same conditions.


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