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Home Antibody All anti-HSPA1B antibodies

Anti-HSPA1B Antibody EP1007Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303852
  • Rabbit monoclonal antibody against Hsp70 (C-term)(EP1007Y)
100ul $325 In Stock
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WB(2)
IHC(1)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-term of human Hsp70 was used as an immunogen.
Clone NameEP1007Y IsotypeIgG
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, FC Suggested DilutionsFC: 1:100 - 1:1000; IP: 1:50; WB: 1:20000; IHC-P: Use at an assay dependent concentration
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for ICC/IF.

Reference Data

Target NameHomo sapiens heat shock 70kDa protein 1B (HSPA1B)
Alternative NameHSP70-1B; HSP70-2
Database LinkNP_005337
FunctionThis intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq].
Related Pathway
MAPK signaling pathway

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WB Image
Western blot - Hsp70 antibody [EP1007Y]; Anti-Hsp70 antibody [EP1007Y] at 1/20000 dilution + HeLa cell lysate at 10 µg.Secondary.Goat anti-rabbit HRP labelled, at 1/2000 dilution.Observed band size : 70 kDa .
WB Image
Western blot - Anti-Hsp70 antibody [EP1007Y]; .Exposure time : 2 minutes
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Hsp70 antibody [EP1007Y]; Ab45133 at a 1/100 dilution staining human Hsp70 in human prostate carcinoma by immunohistochemistry using paraffin embedded tissue.
FC Image
Flow Cytometry - Anti-Hsp70 antibody [EP1007Y]; Overlay histogram showing Jurkat cells stained with TA303852 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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