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Home Antibody All anti-HSPA1A antibodies

Anti-HSPA1A TRUEMAB Antibody Clone OTI1B2

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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SKU Description Amount Price Availability*  
TA500827 anti-HSPA1A (Hsp70) mouse monoclonal antibody, clone OTI1B2 (formerly 1B2) 100ul $325 In Stock
LC401646 HSPA1A HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500827 Carrier-free (BSA/glycerol-free) HSPA1A mouse monoclonal antibody, clone OTI1B2 (formerly 1B2) 100ug $450 3-4 weeks
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WB(2)
IHC(6)
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Also for HSPA1A (NM_005345)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human HSPA1A (NP_005336) produced in HEK293T cell.
Clone NameClone OTI1B2 IsotypeIgG1
Species ReactivityHuman, Dog, Monkey Concentration0.5 mg/ml
Guaranteed Application *WB, IHC Suggested DilutionsWB 1:2000, IHC 1:50,
Predicted MW Explanation 70.0 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens heat shock 70kDa protein 1A (HSPA1A)
Alternative NameHEL-S-103; HSP70-1; HSP70-1A; HSP70I; HSP72; HSPA1
Database LinkNP_005336
Entrez Gene 3303 Human
FunctionThis intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.
Related Pathway SpliceosomeMAPK signaling pathwayEndocytosisAntigen processing and presentationPrion diseases

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA1A (RC200270, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA1A.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HSPA1A monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-HSPA1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500827, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-HSPA1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500827, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-HSPA1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500827, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-HSPA1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500827, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-HSPA1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500827, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-HSPA1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500827, Dilution 1:50)
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