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Anti-HSPA1A TRUEMAB Antibody Clone OTI3C6, Loading Control
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for HSPA1A (NM_005345)
|Full length human recombinant protein of human HSPA1A (NP_005336) produced in HEK293T cell.|
|Human, Dog, Monkey
|WB, IHC, IF, FC
||WB 1:1000 - 2000, IHC 1:50, IF 1:100, FC 1:100
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens heat shock 70kDa protein 1A (HSPA1A)|
|HEL-S-103; HSP70-1; HSP70-1A; HSP70I; HSP72; HSPA1|
Entrez Gene 3303 Human
|This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. |
| SpliceosomeMAPK signaling pathwayEndocytosisAntigen processing and presentationPrion diseases|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA1A (RC200270, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA1A.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HSPA1A monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-HSPA1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500772, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-HSPA1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500772, Dilution 1:50)
Anti-HSPA1A mouse monoclonal antibody (TA500772) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HSPA1A(RC200270).
Flow cytometric Analysis of Hela cells, using anti-HSPA1A antibody(TA500772),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).