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Anti-HSPA1A Antibody 2A4
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Also for HSPA1A (NM_005345)
|Human Recombinant Hsp70 overexpressed in E.coli|
|Human, Mouse, Rat, Amphibian, Chicken, Fish, Saccharomyces cerevisiae, Fruit Fly
||ICC/IF: 1:100, IHC: 1:100, WB: 1:5000
|PBS pH7.2, 50% glycerol|
|Detects several members of the heat shock protein 70kDa gene family including Hsp70, Hsc70, and following heat shock, Hsp72 from yeast, Drosophila, fish, mouse, avian, amphibian and human samples. IF staining of Hsp70 in heat shocked HeLa cells results in cytoplasmic staining.
|Homo sapiens heat shock protein family A (Hsp70) member 1A (HSPA1A)|
|HEL-S-103; HSP70-1; HSP70-1A; HSP70I; HSP72; HSPA1|
Entrez Gene 3303 Human
Entrez Gene 193740 Mouse
Entrez Gene 24472 Rat
|Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity . The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides . When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half . The structure of this ATP binding domain displays multiple features of nucleotide binding proteins .All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein . The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.|
| SpliceosomeMAPK signaling pathwayEndocytosisAntigen processing and presentationPrion diseases|
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Western blot analysis of Hsp70 in rat tissue mix using a 1:5000 dilution of the antibody