OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
Home Antibody All anti-HSPA1A antibodies

Anti-HSPA1A Antibody C92F3A-5


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA326356 Mouse monoclonal Hsp70 Antibody 200µg $325 3-7 Days
Add to Shopping Cart
Write Review & Get Rewarded

Write a review and get rewarded!

Review this antibody and earn an OriGene coupon or Amazon giftcard

Follow these easy steps to reward yourself:

  1. Sign in to your account, or register for a new account;
  2. Write a review;
  3. Receive a coupon or giftcard once your review is accepted.

Click the button to start writing a review

Also for HSPA1A (NM_005345)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenHuman Hsp70
Clone NameC92F3A-5 IsotypeIgG
Species ReactivityHuman, Mouse, Rat, Bovine, C.elegans, Canine, Chicken, Drosophilia, Carp, Guinea pig, Hamster, Monkey, Pig, Rabbit, Sheep Concentration1mg/mL
Guaranteed Application *WB, IHC, IF Suggested Dilutions1ug/ml was sufficient for detection of Hsp70 in 20ug of Hela cell lysate
BufferPBS pH7.2, 50% glycerol, 0.09% sodium azide.
Purification Protein G Purified
Note Detects a ~70kDa protein corresponding to the molecular mass of inducible Hsp70 on SDS PAGE immunoblots. The mapped epitope is in the region of amino acid residues 436-503. Does not cross-react with Hsc70 (Hsp73).

Reference Data

Target NameHomo sapiens heat shock 70kDa protein 1A (HSPA1A)
Alternative NameHEL-S-103; HSP70-1; HSP70-1A; HSP70I; HSP72; HSPA1
Database LinkNP_005336
Entrez Gene 3303 Human
Entrez Gene 193740 Mouse
Entrez Gene 24472 Rat
FunctionHsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity . The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides . When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half . The structure of this ATP binding domain displays multiple features of nucleotide binding proteins . All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein . The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.
Related Pathway SpliceosomeMAPK signaling pathwayEndocytosisAntigen processing and presentationPrion diseases

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot analysis of Hsp70 in cell lysates from 12 human cancer cell lines at 1:1000 dilution of TA326356.
Human colon tissue stained (brown) in inflammatory cells.? Shown at a 1:100,000 dilution.
Mouse melanoma cells heat shocked and probed with C92 (TA326356 was used at 1:1000 dilution in 1% BSA for a final conc. of 10ug/mL).?


Inc 5000 Healthcare Company
All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title

Notice to Non-US Customers

ExclaimationWeb price and delivery time are for direct sales only.

Non-US customers are encouraged to contact our dedicated distributor network for quotes and streamlined ordering/delivery support.