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Home Antibody All anti-HSPA1A antibodies

Anti-HSPA1A Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA308905
  • Rabbit Polyclonal antibody to HSP70 1A (heat shock 70kDa protein 1A)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401646) , 20ug Explanation
100ul 325 In Stock
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WB(4)
IP(1)
IHC(1)
IF(1)
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Also for HSPA1A (NM_005345)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenRecombinant protein fragment contain a sequence corresponding to a region within amino acids 308 and 569 of HSP70 1A
Clone Name IsotypeIgG
Species ReactivityHuman, Mouse (Predicted: Sheep, Bovine) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, IP Suggested DilutionsICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:5000-1:20000
Predicted MW Explanation 70kDa
Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Purification Purified by antigen-affinity chromatography.
Note Seq homology of immunogen across species: Sheep (100%), Bovine (100%)

Reference Data

Target NameHomo sapiens heat shock 70kDa protein 1A (HSPA1A)
Alternative NameHEL-S-103; HSP70-1; HSP70-1A; HSP70I; HSP72; HSPA1
Database LinkNP_005336
Entrez Gene 3303 Human
Entrez Gene 193740 Mouse
FunctionThis intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq]
Related Pathway SpliceosomeMAPK signaling pathwayEndocytosisAntigen processing and presentationPrion diseases

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Sample (whole cell lysate). A: 293T 20ug. B: 293T 10ug. C: 293T 5ug. D: 293T 1ug. 7.5% SDS PAGE. TA308905 diluted at 1:10000.
WB Image
Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. TA308905 diluted at 1:10000.
WB Image
Sample (30 ug of whole cell lysate). A: Jurkat. B: Raji. C: 293T. D: A431. E: HeLa. F: HepG2. G: H1299. H: HCT116. I: MCF-7. J: NT2D1. K: PC-3. L: U87-MG. 7.5% SDS PAGE. TA308905 diluted at 1:10000.
WB Image
Sample (30 ug of whole cell lysate). A: 293T. B: NIH-3T3. C: Mouse brain. D: PC-12. E: Rat brain. 7.5% SDS PAGE. TA308905 diluted at 1:10000.
IHC Image
HSP70 1A antibody detects HSPA1A protein at cytosol on H1299 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded H1299 xenograft. HSP70 1A antibody (TA308905) dilution: 1:500.
IF Image
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using HSP70 1A(TA308905) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2000.
IP Image
HSP70 1A antibody immunoprecipitates HSP70 1A protein in IP experiments. IP Sample: 1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2.5 ug of preimmune rabbit IgG C. Immunoprecipitation of HSP70 1A protein by

 

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