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Anti-HSP90B1 Antibody EPR3988
Also for HSP90B1 (NM_003299)
|A synthetic peptide corresponding to residues in human GRP94 was used as an immunogen.|
||Tissue culture supernatant
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC: 1:50 - 1:100
|Does not react with Rat. Is unsuitable for Flow Cyt or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens heat shock protein 90kDa beta (Grp94), member 1 (HSP90B1)|
|ECGP; GP96; GRP94; HEL-S-125m; HEL35; TRA1|
|The 90-kDa heat shock protein (HSP90) is a highly conserved, and abundant cytosolic homodimeric molecular chaperone (1). HSP90 is distinguished from other chaperones in that most of its known substrates are signal transduction proteins, non activated steroid hormone receptors, several protooncogenic tyrosine and serine/threonine kinases and actin (2-3). GRP94 functions in endoplasmic reticulum associated degradation (ERAD), so it is localized in the endoplasmic reticulum lumen (3).|
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Western blot - GRP94 antibody [EPR3988]; All lanes : Anti-GRP94 antibody [EPR3988] at 1/1000 dilution.Lane 1 : T47D cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : K562 cell lysate.Lane 4 : Jurkat cell lysate.Lane 5 : HepG2 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 92 kDa.Observed band size : 94 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GRP94 antibody [EPR3988]; TA307367, at 1/100 dilution, staining GRP94 in paraffin-embedded Human breast tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GRP94 antibody [EPR3988]; TA307367, at 1/100 dilution, staining GRP94 in paraffin-embedded Human gastric adenocarcinoma tissue by Immunohistochemistry.