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Anti-HSF1 PHOSPHO Antibody EPR1712
Also for HSF1 (NM_005526)
|A phospho specific peptide corresponding to residues surrounding serine 320 of human HSF1 was used as an immunogen. This antibody detects HSF1 phosphorlyated on serine 320.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:10000 - 1:20000; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens heat shock transcription factor 1 (HSF1)|
|The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene. [provided by RefSeq]. |
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Western blot - HSF1 (phospho S320) antibody [EPR1712]; All lanes : Anti-HSF1 (phospho S320) antibody [EPR1712] at 1/20000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : HeLa cell lysate, heat treated at 42°C.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit-HRP at 1/1000 dilution.developed using the ECL technique.Predicted band size : 57 kDa.Observed band size : 90 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HSF1 (phospho S320) antibody [EPR1712]; Immunohistochemical analysis of paraffin-embedded human muscles using ab76183 at 1/100 dilution.