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Anti-HSF1 PHOSPHO Antibody EP1713Y
Also for HSF1 (NM_005526)
|A phospho specific peptide corresponding to residues surrounding Ser326 of human HSF1 was used as an immunogen. This antibody detects HSF1 phosphorylated on Ser326.|
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, FC
||WB: 1:5000 - 1:10000; IP: 1:80; ICC: 1:250 - 1:500; FC: 1:1000; IHC-P: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Does not react with Mouse, Rat
|Homo sapiens heat shock transcription factor 1 (HSF1)|
Entrez Gene 3297 Human
|The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene. [provided by RefSeq]. |
|Transcription Factors |
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Western blot - HSF1 (phospho S326) antibody [EP1713Y]; All lanes : Anti-HSF1 (phospho S326) antibody [EP1713Y] at 1/10000 dilution.Lane 1 : HeLa cell lysates (untreated).Lane 2 : HeLa cell lysates, treated with heat (44o C).Lysates/proteins at 10 ug per lane.Secondary.Goat anti-rabbit HRP at 1/1000 dilution.Predicted band size : 57 kDa.Observed band size : 82 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HSF1 (phospho S326) antibody [EP1713Y]; Immunohistochemical analysis of paraffin-embedded human stomach using TA303540, at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HSF1 (phospho S326) antibody [EP1713Y]; Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using TA303540, at 1/100 dilution.
Flow Cytometry - Anti-HSF1 (phospho S326) antibody; Overlay histogram showing HeLa cells stained with TA303540 (red line). The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1:2000. Isotype control antibody (black line) was rabbit IgG (monoclonal) used under the same conditions. Unlabelled sample (blue line) was also used as a control. This antibody gave a positive signal in HeLa cells under the same conditions.