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Anti-HSF1 Antibody EP1710Y
Also for HSF1 (NM_005526)
|A synthetic peptide corresponding to residues near the C-terminus of human HSF1 was used as an immunogen.|
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, FC
||WB: 1:100000; IP: 1:100; FC: 1:1000; IHC-P: Use at an assay dependent concentration; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Does not react with Mouse, Rat
|Homo sapiens heat shock transcription factor 1 (HSF1)|
Entrez Gene 3297 Human
|The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene. [provided by RefSeq]. |
|Transcription Factors |
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Western blot - HSF1 antibody [EP1710Y]; Anti-HSF1 antibody [EP1710Y] at 1/100000 dilution + Hela cell lysate at 10 ug.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 57 kDa.Observed band size : 85 kDa .
Immunohistochemistry (Paraffin-embedded sections) - HSF1 antibody [EP1710Y]; Immunohistochemical staining of paraffin-embedded human ovarian carcinoma using TA303539 at a 1:100 dilution.
Flow Cytometry - Anti-HSF1 antibody; Overlay histogram showing HeLa cells stained with TA303539 (red line). The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1:2000. Isotype control antibody (black line) was rabbit IgG (monoclonal) used under the same conditions. Unlabelled sample (blue line) was also used as a control. This antibody gave a positive signal in HeLa cells under the same conditions.