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Home Antibody All anti-HRAS antibodies

Anti-HRAS Antibody Y132


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA300461 Rabbit Monoclonal Antibody against HRAS (Clone Y132) 100ul $325 In Stock
LC401645 HRAS HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the C-terminus of human H-Ras was used as an immunogen. The antibody does not cross-react with other Ras family member. Predicted to cross-react with chicken, based on sequence homology.
Clone NameY132 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF, FC Suggested DilutionsWB: 1:500; ICC/IF: 1:100 - 1:250; IP: 1:50; FC: 1:100
Predicted MW Explanation 21 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Protein A purified
Note Is unsuitable for IHC.

Reference Data

Target NameHomo sapiens Harvey rat sarcoma viral oncogene homolog (HRAS), transcript variant 1
Alternative NameC-BAS/HAS; C-H-RAS; C-HA-RAS1; CTLO; H-RASIDX; HAMSV; HRAS1; p21ras; RASH1
Database LinkNP_005334
Entrez Gene 3265 Human
Entrez Gene 15461 Mouse
Entrez Gene 293621 Rat
FunctionRas, a signal transducer, was first characterized as the transforming genes of Harvey & Kristen sarcoma virus (1). The Ras family (H-Ras, N-Ras, and K-Ras) regulates cell growth, differentiation and apoptosis (2). Switching from an active or resting state, Ras can either bind GTP or GDP respectively. In the triphosphate conformation, Ras will interact with GTPase activating protein (GAP) to increase its activity (3). Mutations in any of the three isoforms can convert these proteins into active oncogenes. Additionally, Ras mutations are found in 30 % of all human cancer (4).
Related PathwayDruggable Genome MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwayEndocytosisAxon guidanceVEGF signaling pathwayMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - GTPase HRAS antibody [Y132]; All lanes : Anti-GTPase HRAS antibody [Y132] at 1/500 dilution.Lane 1 : MCF7 cell lysate.Lane 2 : PC12 cell lysate.Observed band size : 21 kDa .
Immunocytochemistry/ Immunofluorescence - GTPase HRAS antibody [Y132]; Immunohistochemical staining of MCF7 cells using TA300461 at 1/100 dilution.
Flow Cytometry - Anti-GTPase HRAS antibody; Overlay histogram showing HeLa cells stained with TA300461 (red line). The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1:2000. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.


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