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Home Antibody All anti-HPRT1 antibodies

Anti-HPRT1 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA308902
  • Rabbit Polyclonal antibody to HPRT (hypoxanthine phosphoribosyltransferase 1)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400070) , 20ug Explanation
100ul 325 In Stock
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WB(2)
IHC(1)
IF(1)
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Also for HPRT1 (NM_000194)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenRecombinant fragment corresponding to a region within amino acids 23 and 218 of HPRT (Uniprot ID#P00492)
Clone Name IsotypeIgG
Species ReactivityHuman (Predicted: Chicken, Dog, Pig, Rabbit, Rat, Xenopus laevis, Zebrafish, Bovine, Xenopus tropicalis) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
Predicted MW Explanation 25kDa
Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Purification Purified by antigen-affinity chromatography.
Note Seq homology of immunogen across species: Chicken (94%), Dog (96%), Pig (97%), Rabbit (98%), Rat (96%), Xenopus laevis (92%), Zebrafish (93%), Bovine (94%), Xenopus tropicalis (92%)

Reference Data

Target NameHomo sapiens hypoxanthine phosphoribosyltransferase 1 (HPRT1)
Alternative NameHGPRT; HPRT
Database LinkNP_000185
Entrez Gene 3251 Human
Entrez Gene 24465 Rat
Entrez Gene 442945 Dog
FunctionHPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. HPRT1 catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate (Keebaugh et al., 2007 [PubMed 16928426]).[supplied by OMIM]
Related PathwayStem cell - PluripotencyDruggable Genome Purine metabolismDrug metabolism - other enzymesMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Sample (30 ug of whole cell lysate). A: Molt-4 . B: Raji. 12% SDS PAGE. TA308902 diluted at 1:1000.
WB Image
HPRT antibody detects HPRT1 protein by Western blot analysis. A. 50 ug mouse liver lysate/extract. 12 % SDS-PAGE. HPRT antibody (TA308902) dilution: 1:500
IHC Image
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using HPRT(TA308902) antibody at 1:100 dilution.
IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HPRT(TA308902) antibody at 1:200 dilution.

 

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