15-Hydroxyprostaglandin dehydrogenase (15-PGDH) is a novel tumor suppressor in the COX-2 pathway. The protein is found in many normal tissues and appears to be down-regulated in colorectal and lung carcinoma tissue.
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HEK293T cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY HPGD (RC204160) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HPGD.