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Anti-HNRNPK Antibody EP943Y
Also for HNRNPK (NM_002140)
|A synthetic peptide corresponding to residues near the N-terminus of human hnRNP was used as an immunogen. This antibody detects both phosphorylated and unphosphorylated hnRNP K.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||WB: 1:100000; IP: 1:50; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens heterogeneous nuclear ribonucleoprotein K (HNRNPK), transcript variant 1|
|CSBP; HNRPK; TUNP|
Entrez Gene 3190 Human
Entrez Gene 15387 Mouse
Entrez Gene 117282 Rat
|The hnRNP-K family of acidic nuclear proteins has been identified using a monoclonal antibody that distinguishes between quiescent and proliferating human keratinocytes. The family, which is composed of four major proteins (hnRNPs-K A, B, C and D) and their modified forms, is present in similar overall levels in quiescent and proliferating normal keratinocytes although clear differences were observed in the levels of some of the individual variants (1). hnRNP-K is an ancient RNA/DNA-binding protein that is involved in multiple processes that compose gene expression. The pleiotropic action of K protein reflects its ability to interact with different classes of factors, interactions that are regulated by extracellular signals (2). hnRNP-K has several different cellular roles including transcription, mRNA shuttling, RNA editing and translation. Several reports implicate hnRNP K having a role in tumorigenesis, for instance hnRNP K increases transcription of the oncogene c-myc and hnRNP K expression is regulated by the p53/MDM 2 pathway (3). |
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Western blot - hnRNP K antibody [EP943Y]; Anti-hnRNP K antibody [EP943Y] at 1/100000 dilution + Jurkat cell lysate at 10 ug.Secondary.HRP-labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 51 kDa.Observed band size : ~60 kDa .
Immunohistochemistry (Paraffin-embedded sections) - hnRNP K antibody [EP943Y]; Immunohistochemical staining of paraffin-embedded human breast carcinoma tissue using TA300758 at a dilution of 1/100-1/250.
Immunocytochemistry/ Immunofluorescence - hnRNP K antibody [EP943Y]; Fluorescent immunostaining of HeLa cells using TA300758 at a dilution of 1/100-1/250.