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Anti-HMGA1 Antibody EPR7839
Also for HMGA1 (NM_002131)
|A synthetic peptide corresponding to residues in human HMGA1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:10000 - 1:50000; IHC-P: 1:250 - 1:500; FC: 1:100 - 1:500; ICC/IF: 1:250 - 1:500
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens high mobility group AT-hook 1 (HMGA1), transcript variant 2|
|HMG-R; HMGA1A; HMGIY|
|HMGA1 is a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. It preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. HMGA1 has little secondary structure in solution, but it assumes distinct conformations when bound to substrates such as DNA or other proteins. It is frequently acetylated and is found in the nucleus (1).|
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Western blot - Anti-HMGA1 antibody [EPR7839]; All lanes : Anti-HMGA1 antibody [EPR7839] at 1/10000 dilution.Lane 1 : SK-OV-3 cell lysate.Lane 2 : Caco-2 cell lysate.Lane 3 : BxPC-3 cell lysate.Lane 4 : HepG2 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 12 kDa.Observed band size : 17 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA1 antibody [EPR7839]; ab129153, at 1/250 dilution, staining HMGA1 in paraffin-embedded Human testis tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-HMGA1 antibody [EPR7839]; ab129153, at 1/250 dilution, staining HMGA1 in BxPC-3 cells by Immunofluorescence.