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UltraMAB

HMBS UltraMAB Antibody, Clone UMAB144

UltraMAB™ Antibodies - Validated with 10K Protein Chip

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Specifications Citations Customer Reviews Product Documents
SKU Description Datasheet Price Availability*  
UM800041
  • HMBS mouse monoclonal antibody,clone UMAB144, 100 ul
$468 In Stock Add to Shopping Cart
UM870041
  • HMBS mouse monoclonal antibody,clone UMAB144, 30 ul
$120 In Stock Add to Shopping Cart
WB(4)
Data Img
IHC(11)
Data Img
IF(1)
Data Img
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OriGene Data

ImmunogenFull length human recombinant protein of human HMBS (NP_000181) produced in HEK293T cell.
Clone NameClone UMAB144 IsotypeIgG1
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF Suggested Dilutions WB 1:2000, IHC 1:200,
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens hydroxymethylbilane synthase (HMBS), transcript variant 1
Alternative NamePBG-D; PBGD; PORC; UPS
Database LinkNP_000181
FunctionThis gene encodes a member of the hydroxymethylbilane synthase superfamily. The encoded protein is the third enzyme of the heme biosynthetic pathway and catalyzes the head to tail condensation of four porphobilinogen molecules into the linear hydroxymethylbilane. Mutations in this gene are associated with the autosomal dominant disease acute intermittent porphyria. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008].
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

Western blot analysis of  HT29 cell lysate (35ug) by using anti-HMBS monoclonal antibody.
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Western blot analysis of  K562 cell lysate (35ug) by using anti-HMBS monoclonal antibody.
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Western blot analysis of KM12 cell lysate (35ug) by using anti-HMBS monoclonal antibody.
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Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:500.
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Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-HMBS mouse monoclonal antibody. (UM800041; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-HMBS mouse monoclonal antibody. (UM800041; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-HMBS mouse monoclonal antibody. (UM800041; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-HMBS mouse monoclonal antibody. (UM800041; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-HMBS mouse monoclonal antibody. (UM800041; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-HMBS mouse monoclonal antibody. (UM800041; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Human muscle tissue using anti-HMBS mouse monoclonal antibody. (UM800041; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue using anti-HMBS mouse monoclonal antibody. (UM800041; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-HMBSmouse monoclonal antibody. (UM800041; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded human tonsil using anti-HMBS clone UMAB144 mouse monoclonal antibody at 1:400 with Polink2 Broad HRP DAB detection kit (UM800041); heat-induced epitope retrieval with citrate buffer, pH6.0at 95-100C. Strong cytoplasmic and membraneous staining is seen on tonsil germinal and non-germinal centers. Some weak nuclear stain was also observed.
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Immunohistochemical staining of paraffin-embedded human prostate cancer using anti-HMBS clone UMAB144 mouse monoclonal antibody at 1:400 with Polink2 Broad HRP DAB detection kit (UM800041); heat-induced epitope retrieval with citrate buffer, pH6.0at 95-100C. Weak nuclear staining is seen on tumor cells.
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Immunofluorescent staining of HeLa cells using anti-HMBS mouse monoclonal antibody (UM800041, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.
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