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Home Antibody All anti-HLA-G antibodies

Anti-HLA-G Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA325084
  • Rabbit polyclonal HLA-G Antibody (Center)
400ul 325 3-7 Days
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WB(3)
IHC(1)
FC(1)
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Also for HLA-G (NM_002127)
cDNA Clone shRNA/siRNA Primer Pair Protein Antibody

OriGene Data

ImmunogenThis HLA-G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-89 amino acids from the Central region of human HLA-G.
Clone Name IsotypeIgG
Species ReactivityHuman Concentration0.3 mg/ml
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:1000, IHC: 1:10~50, FC: 1:10~50
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.

Reference Data

Target NameHomo sapiens major histocompatibility complex, class I, G (HLA-G)
Alternative NameMHC-G
Database LinkNP_002118
Entrez Gene 3135 Human
Function
Related PathwayTransmembrane EndocytosisCell adhesion molecules (CAMs)Antigen processing and presentationNatural killer cell mediated cytotoxicityType I diabetes mellitusAutoimmune thyroid diseaseMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot analysis of HLA-G Antibody (Center) (Cat. #TA325084) in NCI-H460 cell line lysates (35ug/lane). HLA-G (arrow) was detected using the purified Pab.
WB Image
Western blot analysis of HLA-G (arrow) using rabbit polyclonal HLA-G Antibody (Center) (Cat. #TA325084). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-G gene.
WB Image
Western blot analysis of lysate from human placenta tissue lysate, using HLA-G Antibody (Center))(Cat. #TA325084). TA325084 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
IHC Image
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HLA-G Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
FC Image
HLA-G Antibody (Center) (Cat. #TA325084) flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

 

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