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Western blot analysis of HLA-G Antibody (Center) (Cat. #TA325084) in NCI-H460 cell line lysates (35ug/lane). HLA-G (arrow) was detected using the purified Pab.
Western blot analysis of HLA-G (arrow) using rabbit polyclonal HLA-G Antibody (Center) (Cat. #TA325084). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-G gene.
Western blot analysis of lysate from human placenta tissue lysate, using HLA-G Antibody (Center))(Cat. #TA325084). TA325084 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HLA-G Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
HLA-G Antibody (Center) (Cat. #TA325084) flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.