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Anti-HLA-F Antibody EPR6803
Also for HLA-F (NM_001098478)
|A synthetic peptide corresponding to residues in human HLA-F was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, ASSAY, IHC
||WB: 1:1000 - 1:10000; IHC-P: 1:250 - 1:500
|Does not react with Mouse. Is unsuitable for Flow Cyt,ICC/IF or IP.|
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Is unsuitable for Flow Cyt,ICC/IF or IP.
|Homo sapiens major histocompatibility complex, class I, F (HLA-F), transcript variant 3|
|CDA12; HLA-5.4; HLA-CDA12; HLAF|
|HLA-F belongs to the HLA class I heavy chain paralogues. It is a non-classical heavy chain that forms a heterodimer with a beta-2 microglobulin light chain, with the heavy chain anchored in the membrane. Unlike most other HLA heavy chains, this molecule is localized in the endoplasmic reticulum and Golgi apparatus, with a small amount present at the cell surface in some cell types. It contains a divergent peptide-binding groove, and is thought to bind a restricted subset of peptides for immune presentation (1). |
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Western blot - Anti-HLA F antibody [EPR6803]; All lanes : Anti-HLA F antibody [EPR6803] at 1/1000 dilution.Lane 1 : HT1376 cell lysate.Lane 2 : Human tonsil lysate.Lane 3 : JAR cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Standard HRP labelled goat anti-rabbit at 1/2000 dilution.developed using the ECL technique.Predicted band size : 39 kDa.
Other-Anti-HLA F antibody [EPR6803](TA310580); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA F antibody [EPR6803]; TA310580, at a 1/250 dilution, staining HLA F in paraffin-embedded Human tonsil tissue by immunohistochemistry.