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Anti-HK2 TRUEMAB Antibody clone 4C5
TrueMAB Antibodies - Made against Authentic Protein Antigens
|Full length human recombinant protein of human HK2 (NP_000180) produced in HEK293T cell.|
|WB, IHC, IF, FC
||WB 1:1000, IHC 1:50, IF 1:100, Flow 1:100
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens hexokinase 2 (HK2)|
|Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways.This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HK2 (RC209482, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HK2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HK2 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-HK2mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500856, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-HK2mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500856, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded prostate tissue using anti-HK2mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500856, Dilution 1:50)
Anti-HK2 mouse monoclonal antibody (TA500856) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HK2(RC209482).
Immunofluorescent staining of HepG2 cells using anti-HK2 mouse monoclonal antibody (TA500856).
HEK293T cells transfected with either pCMV6-ENTRY HK2(RC209482)(Red) or empty vector control plasmid(Blue) were immunostained with anti-HK2 mouse monoclonal(TA500856), and then analyzed by flow cytometry.