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Home Antibody All anti-HIST3H3 antibodies

Anti-HIST3H3 PHOSPHO Antibody E191

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303746
  • Rabbit monoclonal antibody against Phospho-Histone H3 (S28)(E191) (phospho-specific)
100ul 325 3-7 Days
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WB(1)
IHC(1)
FC(1)
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Also for HIST3H3 (NM_003493)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Ser28 of human Histone H3 was used as immunogen. The antibody only detects Histone H3 phosphorylated on Serine 28.
Clone NameE191 IsotypeIgG
Species ReactivityMouse, Rat, Guinea pig, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:2000; IHC-P: Use at an assay dependent concentration; ICC/IF: 1:250; FC: 1:100; IHC-FoFr: Use at an assay dependent concentration
Predicted MW Explanation 17 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Protein A purified

Reference Data

Target NameHomo sapiens histone cluster 3, H3 (HIST3H3)
Alternative NameH3.4; H3/g; H3FT; H3t
Database LinkNP_003484
Entrez Gene 8290 Human
Entrez Gene 691496 Rat
FunctionHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Related Pathway Systemic lupus erythematosus

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Histone H3 (phospho S28) antibody [E191]; All lanes : Anti-Histone H3 (phospho S28) antibody [E191] at 1/2000 dilution.Lane 1 : NIH 3T3 cell lysate -untreated.Lane 2 : NIH 3T3 cell lysate -treated with FBS + CalA.Predicted band size : 17 kDa.Observed band size : 17 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Histone H3 (phospho S28) antibody [E191]; Immunohistochemical analysis of Histone H3 (phospho S28) expression in paraffin embedded lymphoma tissue sections, using 1/100 TA303746. left: untreated sample; right: phosphatase treated sample (negative control).
FC Image
Flow Cytometry - Anti-Histone H3 (phospho S28) antibody [E191]; Overlay histogram showing HeLa cells stained with TA303746 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

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