A synthetic phospho-peptide corresponding to residues surrounding Ser28 of human Histone H3 was used as immunogen. The antibody only detects Histone H3 phosphorylated on Serine 28.
Buffer
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Related Pathway
EGFR1 Signaling Pathway
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Western blot analysis on NIH3T3 cell lysate using anti- Phospho-Histone H3 (pS28) RabMAb (TA303746); dilution 1:2,000. Cell lysates were (1) untreated, or (2) treated with FBS and CalA.
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