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Anti-HIST2H2BF Antibody EP955Y
Also for HIST2H2BF (NM_001024599)
|A synthetic acetylated peptide corresponding to residues surrounding Lysine 15 of Histone H2B was used as an immunogen. The antibody only detects Histone H2B acetylated on Lysine 15.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||ICC/IF: 1:500; WB: 1:2000 - 1:5000; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens histone cluster 2, H2bf (HIST2H2BF), transcript variant 1|
|FLJ35099; FLJ56780; FLJ56787; MGC131639|
|Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H2B is primarily acetylated at lysines 5, 12, 15, and 20 (3). Ubiquitylation of Histone H2B at lysine 123 by Rad6 has been implicated in transcriptional activation and lysine methylation on histone H3 (4). Histone H2B phosphorylation at Ser10 (for yeast) and Ser14 (for mammalian cells) has been linked to regulation of apoptosis and meiosis (5). |
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Western blot - Histone H2B (acetyl K15) antibody [EP955Y]; All lanes : Anti-Histone H2B (acetyl K15) antibody [EP955Y] at 1/2000 dilution.Lane 1 : HeLa cell lysate (untreated).Lane 2 : HeLa cell lysate (treated with TSA).Lysates/proteins at 10 µg per lane.Secondary.Goat anti-Rabbit HRP conjugate at 1/2000 dilution.Predicted band size : 14 kDa.Observed band size : 17 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H2B (acetyl K15) antibody [EP955Y]; Ab62335 (1/100-1/250) staining human Histone H2B in human breast carcinoma tissue by immunohistochemistry using paraffin embededd tissue.
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K15) antibody [EP955Y]; ab62335 (1/500) staining Histone H2B (acetyl K15) in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see Abreview.