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Anti-HIST2H2BF Antibody EP957Y
Also for HIST2H2BF (NM_001024599)
|A synthetic acetylated peptide corresponding to residues surrounding Histone H2B was used as an immunogen.|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||WB: 1:20000; IP: 1:30; IHC-P: Use at an assay dependent concentration; ICC/IF: 1:50 - 1:100; FC: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens histone cluster 2, H2bf (HIST2H2BF), transcript variant 1|
|FLJ35099; FLJ56780; FLJ56787; MGC131639|
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Western blot - Histone H2B antibody [EP957Y]; Anti-Histone H2B antibody [EP957Y] at 1/20000 dilution + A431 cell lysate at 10 µg.Secondary.HRP-labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 14 kDa.Observed band size : 14 kDa.
Immunohistochemistry (Paraffin-embedded sections) - Histone H2B antibody [EP957Y]; Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using TA303813 at a dilution of 1/250-1/500.
Immunocytochemistry/ Immunofluorescence - Histone H2B antibody [EP957Y]; Fluorescent staining of HeLa cells using TA303813 at a dilution of 1/50-1/100.
Flow Cytometry - Anti-Histone H2B antibody [EP957Y]; Overlay histogram showing HeLa cells stained with TA303813 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22Â°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22Â°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1Âµg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.