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Anti-HIST2H2BF Antibody EP865Y
Also for HIST2H2BF (NM_001024599)
|A synthetic acetylated peptide corresponding to residues surrounding Lys 27 of Histone H3T was used as immunogen. The antibody only detects Histone H3T acetylated on Lysine 27.|
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||WB: 1:500; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100 - 1:250
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens histone cluster 2, H2bf (HIST2H2BF), transcript variant 1|
|FLJ35099; FLJ56780; FLJ56787; MGC131639|
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Western blot - Histone H3T antibody [EP865Y]; All lanes : Anti-Histone H3 (acetyl K27) [EP865Y] antibody at 1/500 dilution.Lane 1 : C6 cell lysate, untreated.Lane 2 : C6 cell lysate, treated with TSA.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 15 kDa.Observed band size : 17 kDa .
Immunohistochemistry (Paraffin-embedded sections) - Histone H3T antibody [EP865Y]; Ab45173 (1:100) staining human Histone H3T in human hepatocellular carcinoma by immunohistochemistry using paraffin embedded tissue.
Immunocytochemistry/ Immunofluorescence - Histone H3T antibody [EP865Y]; Immunofluorescent staining of A431 cells using TA303810 (1:100).