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Home Antibody All anti-HIST2H2BF antibodies

Anti-HIST2H2BF Antibody EP865Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303810
  • Rabbit monoclonal antibody against Histone H3T, Acetyl (K27)(EP865Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC422516) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(1)
IF(1)
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Also for HIST2H2BF (NM_001024599)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic acetylated peptide corresponding to residues surrounding Lys 27 of Histone H3T was used as immunogen. The antibody only detects Histone H3T acetylated on Lysine 27.
Clone NameEP865Y IsotypeIgG
Species ReactivityMouse, Rat, Human, Saccharomyces cerevisiae ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:500; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100 - 1:250
Predicted MW Explanation 15 kDa
BufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purification IgG fraction
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens histone cluster 2, H2bf (HIST2H2BF), transcript variant 1
Alternative NameFLJ35099; FLJ56780; FLJ56787; MGC131639
Database LinkNP_001019770
Entrez Gene 440689 Human
Function
Related Pathway Systemic lupus erythematosus

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Histone H3T antibody [EP865Y]; All lanes : Anti-Histone H3 (acetyl K27) [EP865Y] antibody at 1/500 dilution.Lane 1 : C6 cell lysate, untreated.Lane 2 : C6 cell lysate, treated with TSA.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 15 kDa.Observed band size : 17 kDa .
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Histone H3T antibody [EP865Y]; Ab45173 (1:100) staining human Histone H3T in human hepatocellular carcinoma by immunohistochemistry using paraffin embedded tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - Histone H3T antibody [EP865Y]; Immunofluorescent staining of A431 cells using TA303810 (1:100).

 

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