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Anti-HIST2H2BF Antibody EP959Y
Also for HIST2H2BF (NM_001024599)
|A synthetic acetylated peptide corresponding to residues surrounding Lys18 of Histone H3 was used as immunogen. The antibody only detects Histone H3 acetylated on Lysine 18.|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||ICC/IF: 1:100 - 1:250; WB: 1:500; IHC-P: Use at an assay dependent concentration; FC: 1:100; ChIP: Use at an assay dependent concentration
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens histone cluster 2, H2bf (HIST2H2BF), transcript variant 1|
|FLJ35099; FLJ56780; FLJ56787; MGC131639|
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Western blot - Histone H3 antibody [EP959Y]; All lanes : Anti-Histone H3 (acetyl K18) antibody [EP959Y] at 1/50000 dilution.Lane 1 : C6 cell lysate, untreated.Lane 2 : C6 cell lysate, treated with TSA.Lysates/proteins at 10 µg per lane.Predicted band size : 17 kDa.Observed band size : 17 kDa.Additional bands at : 50 kDa (possible non-specific binding),60 kDa (possible non-specific binding).
Immunohistochemistry (Paraffin-embedded sections) - Histone H3 antibody [EP959Y]; TA303809 diluted 1:100, staining acetylated histone H3 on human breast carcinoma sections.
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K18) antibody [EP959Y]; TA303809 (1/500) staining Histone H3 (acetyl K18) in asynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.
Flow Cytometry - Anti-Histone H3 (acetyl K18) antibody [EP959Y]; Overlay histogram showing HeLa cells stained with TA303809 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22Â°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22Â°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1Âµg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.